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Bioinformatic analysis of glutathione peroxidase family from theobroma cacao and gene expression during Moniliophthora perniciosa infection.[Poster-B185]

Martins Alves Akyla Maria, Pereira Menezes Sara, Peres Gramacho Karina, Andrade Bruno Silva, Micheli Fabienne. 2018. Bioinformatic analysis of glutathione peroxidase family from theobroma cacao and gene expression during Moniliophthora perniciosa infection.[Poster-B185]. In : Abstracts of 12th IPMB2018. Agropolis Fondation, MUSE, l'INRA, le CNRS, Montpellier SupAgro, l'Université de Montpellier, l'IRD, CIRAD, la SFBV, le Labex Tulip, la SPS, Molecular Plant, The Plant Cell, Communications Biology, la Région Occitanie - Pyrénées/Méditerranée. Montpellier : IPMB, Résumé, p. 104. Congress of the International Plant Molecular Biology ( IPMB2018). 12, Montpellier, France, 5 August 2018/10 August 2018.

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Abstract : Glutathione peroxidases (GPXs) are enzymes which are part of the antioxidant system of the cell. Mammalian GPXs are known as selenoproteins because containing the selenocysteine (Sec) amino acid. In plants, these proteins are less known. Here, were analyzed the protein structure and the gene expression of five GPXs from Theobroma cacao . The three-dimensional structure of the TcGPXs showed that the catalytic site of Tc PHGPX and TcGPX ( 2,4 and 5) contain a cysteine while the GPX8 contain a tryptophan. Interestingly, the T. cacao GPX did not show any selenocysteine in their structure. Docking analysis revealed that TcGPXs can bind to selenium. Phylogenetic analysis split plant and mammalian GPXs in two distinct branches. RT-qPCR analysis of TcGPXs during the T. cacao - Moniliophthora perniciosa interaction showed that TcGPX8 gene is overexpressed in the green broom phase of the susceptible cacao variety. In the resistant variety, the TcGPX5 was significantly more expressed in the final stages of the interaction. This study shows that TcGPXs are important targets for the understanding of the T. cacao - M. perniciosa interaction but also for the functionality of these proteins.

Auteurs et affiliations

  • Martins Alves Akyla Maria, UESC (BRA)
  • Pereira Menezes Sara, UESC (BRA)
  • Peres Gramacho Karina, CEPLAC (BRA)
  • Andrade Bruno Silva, UESB (BRA)
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/594942/)

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