The nitrate transporter family protein LjNPF8.6 controls the N-fixing nodule activity

Totev Valkov Vladimir, Rogato Alessandra, Martins Alves Ludovico, Sol Stefano, Noguero Mélanie, Leran Sophie, Lacombe Benoit, Chiurazzi Maurizio. 2017. The nitrate transporter family protein LjNPF8.6 controls the N-fixing nodule activity. Plant Physiology, 175 : pp. 1269-1282.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
Published version - Anglais
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Valkov et al., 2017 The Nitrate Transporter Family Protein LjNPF8.6 Controls the N-Fixing Nodule Activity.pdf

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Quartile : Outlier, Sujet : PLANT SCIENCES

Abstract : N-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with soil bacteria, rhizobia. The nodule functioning is still a poorly characterized step of the symbiotic interaction, as only a few of the genes induced in N-fixing nodules have been functionally characterized. We present here the characterization of a member of the Lotus japonicus nitrate transporter1/peptide transporter family, LjNPF8.6. The phenotypic characterization carried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and increased nodular superoxide content. The partially compromised nodule functioning induces two striking phenotypes: anthocyanin accumulation already displayed 4 weeks after inoculation and shoot biomass deficiency, which is detected by long-term phenotyping. LjNPF8.6 achieves nitrate uptake in Xenopus laevis oocytes at both 0.5 and 30 mm external concentrations, and a possible role as a nitrate transporter in the control of N-fixing nodule activity is discussed.

Mots-clés Agrovoc : Fixation de l'azote, Bactérie fixatrice de l'azote, Rhizobium, Lotus, Formation de nodosités, nitrogen fixation genes [EN], Xenopus laevis, Nitrate

Mots-clés complémentaires : Lotus japonicus

Mots-clés libres : Nitrogen transporter, Nodulation, Lotus japonicus

Classification Agris : P34 - Soil biology
P35 - Soil fertility

Champ stratégique Cirad : Hors axes (2014-2018)

Auteurs et affiliations

  • Totev Valkov Vladimir, IBBR (ITA)
  • Rogato Alessandra, IBBR (ITA)
  • Martins Alves Ludovico, IBBR (ITA)
  • Sol Stefano, IBBR (ITA)
  • Noguero Mélanie, CNRS (FRA)
  • Leran Sophie, CIRAD-BIOS-UMR IPME (FRA)
  • Lacombe Benoit, CNRS (FRA)
  • Chiurazzi Maurizio, IBBR (ITA) - auteur correspondant

Source : Cirad-Agritrop (

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