Functional characterization of the arabidopsis abscisic acid transporters NPF4.5 and NPF4.6 in xenopus oocytes

Leran Sophie, Noguero Mélanie, Corratgé-Faillie Claire, Boursiac Yann, Brachet Chantal, Lacombe Benoit. 2020. Functional characterization of the arabidopsis abscisic acid transporters NPF4.5 and NPF4.6 in xenopus oocytes. Frontiers in Plant Science, 11:144, 6 p.

https://doi.org/10.3389/fpls.2020.00144

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Functional characterization of the Arabidopsis Abscisic acid transporters NPF4.4 and NPF4.6 in Xenopus oocytes, Léran et al., 2020.pdf
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Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Few proteins have been characterized as abscisic acid transporters. Several of them are NRT1/PRT Family (NPF) transporters which have been characterized in yeast using reporter systems. Because several members of the NPF4 subfamily members were identified in yeast as ABA transporters, here, we screened for ABA transport activity the seven members of the NPF4 subfamily in Xenopus oocytes using cRNA injection and 3H-ABA accumulation. The ABA transport capacities of NPF4.2, NPF4.5, NPF4.6, and NPF4.7 were confirmed. The transport properties of NPF4.5 and NPF4.6 were studied in more detail. Both ABA transporter activities are pH-dependent and slightly pH-dependent apparent Km around 500 μM. There is no competitive inhibition of the ABA-analogs pyrabactin and quinabactin on ABA accumulation demonstrating a different selectivity compared to the ABA receptors. Functional expression of these ABA transporters in Xenopus oocyte is an opportunity to start structure–function studies and also to identify partner proteins of these hormone transporters.

Mots-clés Agrovoc : Arabidopsis thaliana, ABA

Mots-clés complémentaires : Xenopus oocytes

Mots-clés libres : Arabidopsis thaliana, NPF transporters, ABA (Abscisic acid), Hormones

Classification Agris : F60 - Physiologie et biochimie végétale
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

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