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Sex determination in the GIFT strain of tilapia is controlled by a locus in linkage group 23

Taslima Khanam, Wehner Stefanie, Taggart John B., De Verdal Hugues, Benzie John A.H., Bekaert Michaël, McAndrew Brendan J., Penman David J.. 2020. Sex determination in the GIFT strain of tilapia is controlled by a locus in linkage group 23. BMC Genetics, 21:49, 15 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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[17] Taslima et al 2020 BMC Genetics sex determination in GIFT.pdf

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Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Abstract : Background: Tilapias (Family Cichlidae) are the second most important group of aquaculture species in the world. They have been the subject of much research on sex determination due to problems caused by early maturation in culture and their complex sex-determining systems. Different sex-determining loci (linkage group 1, 20 and 23) have been detected in various tilapia stocks. The 'genetically improved farmed tilapia' (GIFT) stock, founded from multiple Nile tilapia (Oreochromis niloticus) populations, with some likely to have been introgressed with O. mossambicus, is a key resource for tilapia aquaculture. The sex-determining mechanism in the GIFT stock was unknown, but potentially complicated due to its multiple origins. Results: A bulk segregant analysis (BSA) version of double-digest restriction-site associated DNA sequencing (BSA-ddRADseq) was developed and used to detect and position sex-linked single nucleotide polymorphism (SNP) markers in 19 families from the GIFT strain breeding nucleus and two Stirling families as controls (a single XY locus had been previously mapped to LG1 in the latter). About 1500 SNPs per family were detected across the genome. Phenotypic sex in Stirling families showed strong association with LG1, whereas only SNPs located in LG23 showed clear association with sex in the majority of the GIFT families. No other genomic regions linked to sex determination were apparent. This region was validated using a series of LG23-specific DNA markers (five SNPs with highest association to sex from this study, the LG23 sex-associated microsatellite UNH898 and ARO172, and the recently isolated amhy marker for individual fish (n = 284). Conclusions: Perhaps surprisingly given its multiple origins, sex determination in the GIFT strain breeding nucleus was associated only with a locus in LG23. BSA-ddRADseq allowed cost-effective analysis of multiple families, strengthening this conclusion. This technique has potential to be applied to other complex traits. The sex-linked SNP markers identified will be useful for potential marker-assisted selection (MAS) to control sex-ratio in GIFT tilapia to suppress unwanted reproduction during growout.

Mots-clés Agrovoc : Tilapia, Cichlidae, Détermination du sexe, Sex ratio, amélioration génétique

Mots-clés libres : Sex determination, GIFT, BSA-ddRADseq, MAS, Sex-ratio control

Classification Agris : L10 - Animal genetics and breeding
L40 - Animal structure

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Auteurs et affiliations

  • Taslima Khanam, University of Stirling (GBR)
  • Wehner Stefanie, University of Stirling (GBR)
  • Taggart John B., University of Stirling (GBR)
  • De Verdal Hugues, CIRAD-PERSYST-UMR ISEM (FRA) ORCID: 0000-0002-1923-8575
  • Benzie John A.H., UCC (IRL)
  • Bekaert Michaël, University of Stirling (GBR)
  • McAndrew Brendan J., University of Stirling (GBR)
  • Penman David J., University of Stirling (GBR) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595679/)

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