Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies

Comerlato Juliana, Albina Emmanuel, Puech Carinne, Franco Ana Cláudia, Minet Cécile, Eloiflin Roger-Junior, Rodrigues Valérie, Servan de Almeida Renata. 2020. Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies. Virus Research, 286:198035, 8 p.

https://doi.org/10.1016/j.virusres.2020.198035

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Quartile : Q3, Sujet : VIROLOGY

Résumé : Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different susceptibility to virulent and attenuated PPRV strains. As evidenced by immunofluorescence test and RT-PCR, both virulent and attenuated PPR viruses penetrated and initiated the replication cycle in 10T1/2 cells, independently of the presence of the SLAM goat receptor. However, only virulent strains successfully completed their replication cycle while the vaccine strains did not. Since 10T1/2 cells are interferon-producing cells, the role of the type I interferon (type I IFN) response on this differentiated replication between virulent and attenuated strains was verified by stimulation or repression. Modulation of the type I IFN response did not improve the replication of the vaccine strains, indicating that other cell factor(s) not yet established may hinder the replication of attenuated PPRV in 10T1/2. This 10T1/2 cell line can be proposed as a new in vitro tool for PPRV-host interaction and virulence studies.

Mots-clés Agrovoc : virus peste petits ruminants, relation hôte pathogène, morbillivirus, virulence, virus pathogène, pathogénèse, peste des petits ruminants, Murinae

Mots-clés libres : PPRV (Peste des Petits Ruminants virus), 10T1/2 cells, Cell permissiveness, SLAM receptor, Type I IFN, Virus-host interaction

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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