Optimising non-destructive sampling methods to study nitrogen use efficiency throughout the growth-cycle of giant C4 crops

Poultney Daniel, Christina Mathias, Versini Antoine. 2020. Optimising non-destructive sampling methods to study nitrogen use efficiency throughout the growth-cycle of giant C4 crops. Plant and Soil, 613 : 453-597.

Url - éditeur : https://rdcu.be/b6gft

Quartile : Q1, Sujet : AGRONOMY / Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : SOIL SCIENCE

Résumé : Aims: The improvement of nitrogen use efficiency (NUE) of crops allows crop nitrogen (N) demands to be met while reducing N supply, and so reducing excess N which has potential negative environmental implications. NUE is often determined destructively at the end of crop growth-cycles without considering temporal variability. Here we present a methodological study which optimises the determination of NUE throughout the sugarcane growth-cycle using minimally destructive methods, and suggest the use of these methods for non-destructive NUE determination over the growth-cycle of other giant C4 crops. Methods and results: The determination of the NUE relied on the optimisation of three methods: the estimation of aboveground biomass, N content and N derived from fertiliser (NdfF). First, the ability of different allometric relationships to estimate sugarcane biomass was investigated by selecting a relationship based on height and diameter to estimate aboveground biomass along the crop growth-cycle. Secondly, we assessed the minimum number of harvested sugarcane required to construct a dilution curve to predict N content from biomass and found that a sampling of 5 sugarcane at 3 dates was sufficient to represent aboveground N content over the growth-cycle. Finally, the ability of 15N content of individual leaves to represent the NdfF in 15N-fertilised cane was tested. The first and second leaf below the top visible dewlap were the most representative. Based on a variance analysis, we assessed the level of influence of each method on the NUE calculation. Crop age accounted for 54% of the variance of NUE, the choice of 15N leaf 13%, with the choice of model to estimate biomass and the number of plants harvested for the N dilution curve, each accounting for less than 2% over the four sampling dates. Conclusions: This study highlighted the importance of evaluating NUE not only at the point of harvest. We propose, therefore, a set of methods to study NUE throughout the sugarcane growth-cycle by using minimally destructive sampling.

Mots-clés Agrovoc : échantillonnage, plante en c4, efficacité d'utilisation, azote, Saccharum

Mots-clés complémentaires : Utilisation de l'azote

Mots-clés libres : Allometric relationship, Dilution curves, Nitrogen use efficiency, 15N labelling, N-fertiliser recovery efficiency, SUGARCANE

Classification Agris : F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement européennes : European Commission

Auteurs et affiliations

• Poultney Daniel, CIRAD-PERSYST-UPR Recyclage et risque (REU)
• Christina Mathias, CIRAD-PERSYST-UPR AIDA (REU) ORCID: 0000-0003-3618-756X
• Versini Antoine, CIRAD-PERSYST-UPR Recyclage et risque (REU) ORCID: 0000-0002-5119-0567 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/596342/)

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