Identification of 'Candidatus Phytoplasma' species in “huanglongbing” infected citrus orchards in the Caribbean

Luis-Pantoja Maritza, Paredes-Tomás Camilo, Uneau Youri, Myrie Wayne, Morillon Raphaël, Satta Eleonora, Contaldo Nicoletta, Pacini Francesco, Bertaccini Assunta. 2021. Identification of 'Candidatus Phytoplasma' species in “huanglongbing” infected citrus orchards in the Caribbean. European Journal of Plant Pathology, 160 (2) : 185-198.

Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : HORTICULTURE / Quartile : Q2, Sujet : PLANT SCIENCES

Résumé : “Huanglongbing” (HLB) is one of the most devastating diseases of citrus orchards worldwide. Samples from 183 citrus plants of different cultivars and rootstock/cultivar combinations, showing HLB symptoms in three Caribbean countries (Cuba, Jamaica, and Guadeloupe-France), were collected to verify the possible co-infection of 'Candidatus Phytoplasma' and 'Candidatus Liberibacter' species. The 64% of the samples resulted positive to the 'Ca. L. asiaticus' and the 27% to diverse 'Ca. Phytoplasma'-related species, moreover about the 14% of the samples infected with 'Ca. Liberibacter' were also found positive to phytoplasmas, indicating the presence of mixed infection especially in the orchards located in Cuba. Moreover, in one of the samples from Jamaica mixed phytoplasma infection was detected. Moreover the detection of only phytoplasmas in 11 symptomatic citrus samples collected from Cuba and Guadeloupe without 'Ca. Liberibacter' detection, confirmed that the symptomatology cannot be the sole criterium to discriminate between the presence of the two pathogens, and molecular detection is necessary to identify single or mixed infections. Diaphorina citri insects collected from Cuba and Guadeloupe resulted infected with 'Ca. L. asiaticus' confirming its active role in the dissemination of the pathogen. Only one insect of the Cicadidae family, collected in Guadeloupe, was found positive for phytoplasma presence. Considering that the phytoplasmas belonging to some 'Candidatus species' were detected in the three countries in different citrus varieties, a relevant role as phytoplasma reservoir can be attribute to citrus orchards.

Mots-clés Agrovoc : Citrus, maladie des plantes, maladie bactérienne, phytoplasme, Diaphorina citri, bactérie gram négatif

Mots-clés géographiques Agrovoc : Cuba, Jamaïque, Guadeloupe, France

Mots-clés complémentaires : maladie du Huanglongbing (HLB), Candidatus liberibacter spp., Candidatus liberibacter asiaticus, Candidatus phytoplasma

Mots-clés libres : Citrus greening, Phloematic bacteria, Molecular detection, Mixed infection, Insect vectors

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Programme de financement européen : H2020

Projets sur financement : (EU) Insect-borne prokaryote-associated diseases in tropical and subtropical perennial crops

Auteurs et affiliations

• Luis-Pantoja Maritza, Research Institute of Tropical Fruit Crops (CUB)
• Paredes-Tomás Camilo, Research Institute of Tropical Fruit Crops (CUB)
• Uneau Youri, ASSOFWI (GLP)
• Myrie Wayne, Coconut Industry Board (JAM)
• Morillon Raphaël, CIRAD-BIOS-UMR AGAP (GLP)
• Satta Eleonora, University of Bologna (ITA)
• Contaldo Nicoletta, University of Bologna (ITA)
• Pacini Francesco, University of Bologna (ITA)
• Bertaccini Assunta, University of Bologna (ITA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597634/)

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