Ethylene response factors regulate expression of HbSUT3, the sucrose influx carrier in laticifers of Hevea brasiliensis

Zhang Yi, Xin Lusheng, Pirrello Julien, Fang Yongjun, Yang Jianghua, Qi Jiyan, Montoro Pascal, Tang Chaorong. 2021. Ethylene response factors regulate expression of HbSUT3, the sucrose influx carrier in laticifers of Hevea brasiliensis. Tree Physiology, 41 (7) : 1278-1288., 11 p.

https://doi.org/10.1093/treephys/tpaa179

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

Prévisualisation

Version Online first - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
Zhang_2021_Hevea_SUT3.pdf
Télécharger (1MB) | Prévisualisation

Prévisualisation

Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
598354.pdf
Télécharger (1MB) | Prévisualisation

Quartile : Q1, Sujet : FORESTRY

Résumé : Natural rubber is an important industrial raw material and is commercially produced by rubber trees (Hevea brasiliensis). The sucrose transporter HbSUT3 plays an essential role in rubber production. Its expression in latex (cytoplasm of rubber-producing laticifers) is induced by bark treatment with Ethrel, an ethylene releaser, and the inducing effect correlates well with Ethrel-stimulated rubber yield increase. However, the mechanisms of ethylene induction on HbSUT3 expression are not known. Here, five Ethylene Response Factor (ERF) genes were identified from the cDNA library of Hevea latex by yeast one-hybrid screening with the promoter of HbSUT3 gene as bait. As revealed in a tobacco (Nicotiana tabacum) protoplast transient expression system, these HbERFs were mainly localized in the nucleus and four of them exhibited apparent transactivation activity. Of the five HbERF genes, HbERF-IXc4 was the most frequently screened in yeast one-hybrid, accounting for 65% of the ERF clones obtained. Moreover, among the five HbERFs, HbERF-IXc4 showed the strongest transactivation capacity when expressed in tobacco protoplast, the highest transcript abundance in latex and a close expressional correlation with its target gene, HbSUT3, in response to the Ethrel treatment. Taken together, our results indicate that ERFs, especially HbERF-IXc4, are critically involved in the activation of HbSUT3 expression in latex after Ethrel treatment on Hevea bark, and thus the stimulated latex yield.

Mots-clés Agrovoc : phytogénétique, physiologie végétale, éthylène, Hevea brasiliensis, saccharose, facteur de transcription, expression des gènes

Mots-clés géographiques Agrovoc : Chine

Mots-clés libres : Ethylene signaling, Gene expression, Rubber biosynthesis, Sucrose transporter, Transcription factor

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations