Taking shortcuts: Investigating the role of small non-coding RNAs during in vitro tissue culture of the oil palm

Martineau Justine, Summo Marilyne, Arraiza Ribera Júlia, Beulé Thierry, Jacob Florence, Leclercq Julie, Guyot Romain, Lopez David, Jaligot Estelle. 2021. Taking shortcuts: Investigating the role of small non-coding RNAs during in vitro tissue culture of the oil palm. . s.l. : s.n., 1 p. EpiCATCH COST Action Conference on "Epigenetic Mechanisms of Crop Adaptation to Climate Change". 1, Florence, Italie, 12 Juillet 2021/14 Juillet 2021.

Matériel d'accompagnement : 1 diaporama (17 vues)

Résumé : The implementation of in vitro tissue culture propagation methods, such as somatic embryogenesis (SE), rely on the amenability of plant cells to undergoing induced reprogrammation (de-differentiation followed by re-differentiation) after treatment by growth regulators. It has been well documented in various species that these processes involve epigenetic regulation pathways that are similar to those associated with responses to abiotic stresses, which makes in vitro-derived material a great system for studying them within (theoretically) genetically homogeneous populations. Originally, our work on SE-derived oil palms stems from the practical necessity of understanding and preventing the emergence of a pervasive somaclonal variation (the mantled floral phenotype) with negative impact on oil production. In doing so, we also aim to leverage the knowledge acquired from this case study to address broader scientific questions related to stress memory and acclimation in perennial species. With this dual objective in mind, we are currently assessing the expression of small non-coding RNAs (sncRNAs) in embryogenic suspensions and their possible roles in the regulation of genome expression and structure. We are using high-throughput transcriptome, small RNAome and degradome datasets to identify both source and target sequences of sncRNA-mediated regulations, and annotate the biological pathways that are affected by these regulations. As our analyses are still under way, we will present a global outline of our project and report on both our progress and a few lessons learned.

Mots-clés libres : Epigenetics, Abiotic stress response, In vitro tissue culture, Oil palm, Small RNAs

Auteurs et affiliations

• Martineau Justine, INRAE (FRA)
• Summo Marilyne, CIRAD-BIOS-UMR AGAP (FRA)
• Arraiza Ribera Júlia, IRD (FRA)
• Beulé Thierry, CIRAD-BIOS-UMR DIADE (FRA)
• Jacob Florence, PalmElit (FRA)
• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
• Guyot Romain, IRD (FRA)
• Lopez David, CIRAD-BIOS-UMR AGAP (FRA)
• Jaligot Estelle, CIRAD-BIOS-UMR DIADE (FRA) ORCID: 0000-0002-9252-5271 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598765/)

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