An HRM assay to differentiate sheeppox virus vaccine strains from sheeppox virus field isolates and other capripoxvirus species

Chibssa Tesfaye Rufael, Settypalli Tirumala Bharani Kumar, Berguido Francisco, Grabherr Reingard, Loitsch Angelika, Tuppurainen Eeva, Nwankpa Nick, Tounkara Karim, Madani Hafsa, Omani Amel, Diop Mariame, Cattoli Giovanni, Diallo Adama, Lamien Charles Euloge. 2019. An HRM assay to differentiate sheeppox virus vaccine strains from sheeppox virus field isolates and other capripoxvirus species. Scientific Reports, 9:6646, 9 p.

https://doi.org/10.1038/s41598-019-43158-x

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/MK005931

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Note générale : Les jeux de données ont été déposés dans la base GenBank under accession numbers MK005931 to MK005967.

Résumé : Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates.

Mots-clés Agrovoc : capripoxvirus, vaccin vivant, mutation, analyse qualitative, diagnostic différentiel, virus de la dermatose nodulaire contagieuse, virus de la variole ovine

Mots-clés complémentaires : Virus de la variole caprine, détection de mutations, High- Resolution Melting (HRM)

Mots-clés libres : PCR-based techniques, Pox virus

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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