In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses

Licciardi Séverine, Loire Etienne, Cardinale Eric, Gislard Marie, Dubois Emeric, Cetre-Sossah Catherine. 2020. In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses. Parasites and Vectors, 13 (1):395, 10 p.

https://doi.org/10.1186/s13071-020-04253-5

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
599682.pdf
Télécharger (2MB) | Prévisualisation

Url - autres données associées : https://github.com/loire/CCS_RNAseq_analysis

Quartile : Q1, Sujet : PARASITOLOGY / Quartile : Q1, Sujet : TROPICAL MEDICINE

Résumé : Background: Arthropod borne virus infections are the cause of severe emerging diseases. Among the diseases due to arboviruses, dengue (DEN) and Rift Valley fever (RVF) are in the top ten in the list of diseases responsible of severe human cases worldwide. Understanding the effects of viral infection on gene expression in competent vectors is a challenge for the development of early diagnostic tools and may enable researchers and policy makers to better anticipate outbreaks in the next future. Methods: In this study, alterations in gene expression across the entire Aedes aegypti genome during infection with DENV and RVFV were investigated in vitro at two time points of infection, the early phase (24 h) and the late phase (6 days) of infection using the RNA sequencing approach Results: A total of 10 upregulated genes that share a similar expression profile during infection with both viruses at early and late phases of infection were identified. Family B and D clip-domain serine proteases (CLIP) were clearly overrepresented as well as C-type lectins and transferrin. Conclusions: Our data highlight the presence of 10 viral genes upregulated in Ae. aegypti during infection. They may also be targeted in the case of the development of broad-spectrum anti-viral diagnostic tools focusing the mosquito vectors rather than the mammalian hosts as they may predict the emergence of outbreaks.

Mots-clés Agrovoc : épidémiologie, virologie, expression des gènes, Aedes aegypti, vecteur de maladie, Virus de la fièvre de la vallée du Rift, arbovirus, transcriptome

Mots-clés complémentaires : Virus de la dengue

Mots-clés libres : In vitro, Transcriptomic responses, Aedes aegypti, Dengue, Rift Valley fever

Classification Agris : S50 - Santé humaine
L72 - Organismes nuisibles des animaux
L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations