Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans

Yang Yin-Shan, Fernandez Bernard, Lagorce Arnaud, Aloin Valérie, Montet De Guillen Karine, Boyer Jean-Baptiste, Dedieu Alain, Confalonieri Fabrice, Armengaud Jean, Roumestand Christian. 2015. Prioritizing targets for structural biology through the lens of proteomics: The archaeal protein TGAM_1934 from Thermococcus gammatolerans. Proteomics, 15 (1) : 114-123.

Url - jeu de données - Entrepôt autre : https://doi.org/10.6019/PXD000402

Quartile : Q1, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : ORFans are hypothetical proteins lacking any significant sequence similarity with other proteins. Here, we highlighted by quantitative proteomics the TGAM_1934 ORFan from the hyperradioresistant Thermococcus gammatolerans archaeon as one of the most abundant hypothetical proteins. This protein has been selected as a priority target for structure determination on the basis of its abundance in three cellular conditions. Its solution structure has been determined using multidimensional heteronuclear NMR spectroscopy. TGAM_1934 displays an original fold, although sharing some similarities with the 3D structure of the bacterial ortholog of frataxin, CyaY, a protein conserved in bacteria and eukaryotes and involved in iron–sulfur cluster biogenesis. These results highlight the potential of structural proteomics in prioritizing ORFan targets for structure determination based on quantitative proteomics data. The proteomic data and structure coordinates have been deposited to the ProteomeXchange with identifier PXD000402 (http://proteomecentral.proteomexchange.org/dataset/PXD000402) and Protein Data Bank under the accession number 2mcf, respectively.

Mots-clés Agrovoc : spectroscopie RMN, protéine, protéine bactérienne, protéomique quantitative, Bacteria

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Genome annotation, High-throughput proteomics, Microbiology, NMR structure, ORFan, Protein evolution

Auteurs et affiliations

• Yang Yin-Shan, Université de Montpellier (FRA)
• Fernandez Bernard, CIRAD-BIOS-UMR CMAEE (FRA)
• Lagorce Arnaud, Université Paris-Sud (FRA)
• Aloin Valérie, CEA (FRA)
• Montet De Guillen Karine, Université de Montpellier (FRA)
• Boyer Jean-Baptiste, CEA (FRA)
• Dedieu Alain, CEA (FRA)
• Confalonieri Fabrice, Université Paris-Sud (FRA)
• Armengaud Jean, CEA (FRA) - auteur correspondant
• Roumestand Christian, Université de Montpellier (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600068/)

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