Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division

Caillaud Marie-Cécile, Paganelli Laetitia, Lecomte Philippe, Deslandes Laurent, Quentin Michaël, Pecrix Yann, Le Bris Manuel, Marfaing Nicolas, Abad Pierre, Favery Bruno. 2009. Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. PloS One, 4 (8):e6757, 9 p.

Quartile : Q1, Sujet : BIOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : Background: In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle. Methodology/Principal Findings: We investigated the mechanism underlying this surveillance mechanism in plants, by characterising the orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed that the cell cycle-regulated BUBR1, BUB3.1 and MAD2 proteins interacted physically with each other. Furthermore, BUBR1 and MAD2 interacted specifically at chromocenters. Following SAC activation by global defects in spindle assembly, these three interacting partners localised to unattached kinetochores. In addition, in cases of 'wait anaphase', plant SAC proteins were associated with both kinetochores and kinetochore microtubules. Unexpectedly, BUB3.1 was also found in the phragmoplast midline during the final step of cell division in plants. Conclusions/Significance: We conclude that plant BUBR1, BUB3.1 and MAD2 proteins may have the SAC protein functions conserved from yeast to humans. The association of BUB3.1 with both unattached kinetochore and phragmoplast suggests that in plant, BUB3.1 may have other roles beyond the spindle assembly checkpoint itself. Finally, this study of the SAC dynamics pinpoints uncharacterised roles of this surveillance mechanism in plant cell division.

Mots-clés libres : Arabidopsis thaliana, Saccharomyces cerevisiae, Nicotiana, Cell fusion, Metaphase, Yeast, Cell cycle and cell division, Mitosis

Auteurs et affiliations

• Caillaud Marie-Cécile, INRA (FRA)
• Paganelli Laetitia, INRA (FRA)
• Lecomte Philippe, INRA (FRA) ORCID: 0000-0003-1040-7886
• Deslandes Laurent, INRA (FRA)
• Quentin Michaël, INRA (FRA)
• Pecrix Yann, Institut méditerranéen d'écologie et de paléoécologie (FRA) ORCID: 0000-0002-6537-3145
• Le Bris Manuel, Institut méditerranéen d'écologie et de paléoécologie (FRA)
• Marfaing Nicolas, INRA (FRA)
• Abad Pierre, INRA (FRA)
• Favery Bruno, INRA (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600450/)

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