Cupuassu (Theobroma grandiflorum [Willd. ex Sprengel] Schumann) fruit development: Key genes involved in primary metabolism and stress response

Ferraz dos Santos Lucas, Santana Silva Raner José, Ludke Falcão Loeni, Moysés Alves Rafael, Marcellino Lucilia Helena, Micheli Fabienne. 2022. Cupuassu (Theobroma grandiflorum [Willd. ex Sprengel] Schumann) fruit development: Key genes involved in primary metabolism and stress response. Agronomy (Basel), 12 (4):763, 15 p.

Résumé : Cupuassu (Theobroma grandiflorum [Willd. ex Sprengel] Schumann) seeds constitute the raw material for oil extraction and fabrication of cupulate (product similar to chocolate). However, fungal diseases such as witches' broom caused by Moniliophthora perniciosa have interfered with the large-scale development of cupuassu plantations. Cupuassu genetic breeding programmes focus on a variety of biotechnological tools or approaches to select genes related to quality or resistance mechanisms. In this study, we used expression and interactomics analyses of preselected genes involved in fruit quality and/or resistance to better understand the molecular and physiological mechanisms associated with these plant processes. It was found that (i) resistant and susceptible cupuassu genotypes showed different pulp characteristics as well as gene expression patterns; (ii) monosaccharide and carbohydrate transport pathways were enhanced during fruit maturation; (iii) sugar accumulation participated in signal transduction associated with fruit development and stress response in maturing fruits; and (iv) maturing pulp and seeds showed increased phospholipid metabolism and translocation, as well as immune system activation. The TgSTP1, TgWRKY33, TgCZF1, and TgUBA1 genes in cupuassu and the orthologues of DIN10, CNI1, and TET8 identified by the interactomics approach may be good candidates for marker-assisted selection in breeding programmes focusing on both fruit quality and resistance/tolerance to biotic/abiotic stress.

Mots-clés Agrovoc : résistance aux maladies, résistance physiologique au stress, réponse de la plante, amélioration des plantes, amélioration génétique, fructification, fruit (botanique), biotechnologie végétale, mécanisme de défense, Theobroma grandiflorum

Mots-clés complémentaires : Moniliophthora perniciosa

Mots-clés libres : Theobroma grandiflorum, Fruit quality, Moniliophthora perniciosa, Interactomics, Signal transduction

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement
H01 - Protection des végétaux - Considérations générales
H50 - Troubles divers des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo à Pesquisa do Estado da Bahia, Empresa Brasileira de Pesquisa Agropecuária

Auteurs et affiliations

• Ferraz dos Santos Lucas, UESC (BRA)
• Santana Silva Raner José, UESC (BRA)
• Ludke Falcão Loeni, EMBRAPA (BRA)
• Moysés Alves Rafael, EMBRAPA (BRA)
• Marcellino Lucilia Helena, EMBRAPA (BRA)
• Micheli Fabienne, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/600578/)

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