Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus

Duong Benoit, Marraccini Pierre, Etienne Hervé, Villain Luc, Hoang Thi Giang, Khong Ngan Giang, Lebrun Michel, Duponnois Robin. 2022. Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus. Biocontrol Science and Technology, 32 (8) : 971-988.

https://doi.org/10.1080/09583157.2022.2070600

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : Bacillus cereus sensu lato strain CCBLR15 is highly efficient in inducing in vitro the mortality of coffee-parasitic nematodes, including Pratylenchus coffeae and Radopholus duriophilus. In the present study, this strain was further characterised to investigate (1) its taxonomic position, (2) the secreted/non-secreted nature of its nematicidal compounds, and (3) its in planta biocontrol capacity towards R. duriophilus. Firstly, analysis of 16S rRNA gene sequences and motility test indicated that CCBLR15 was closely related to B. cereus sensu stricto. Secondly, nematodes separate expositions in vitro to CCBLR15 culture filtrate and cell lysate revealed that nematicidal activity was attributed to compounds released after bacterial cell lysis. Thirdly, CCBLR15 nematode biocontrol capacity was studied using three Coffea arabica cultivars, either seed- or cutting-propagated. Plants were therefore characterised by different developmental stages, referred to as 'early' and 'advanced', with respectively 2-3 and 6-8 leaf pairs. Additionally, plants were grown in unsterilised soil, with a one-month delay between inoculations of bacteria and nematodes to assess the persistence of the nematicidal effects. The presence of R. duriophilus in roots decreased plant growth only in one cultivar whose plants were inoculated at the 'early' stage. Nonetheless, CCBLR15 pre-inoculation significantly reduced the number of nematodes in roots of all cultivars and inhibited the negative effects of nematodes in 'early'-inoculated plants. These results demonstrate the capacity of CCBLR15 to control R. duriophilus and constitute an important step before studying the persistence of its biocontrol effects on a longer period or after plants establishment in the field.

Mots-clés Agrovoc : lutte antinématode, endophyte, nématode des plantes, nématocide, Bacillus cereus, protection biologique des plantes, Coffea arabica

Mots-clés complémentaires : Radopholus duriophilus

Mots-clés libres : Coffee, Nematicidal activity, Bacillus cereus sensu lato, Biocontrol, Coffea arabica, Endophyte, Nematodes

Classification Agris : H01 - Protection des végétaux - Considérations générales
H50 - Troubles divers des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Agences de financement hors UE : Institut de Recherche pour le Développement

Programme de financement européen : H2020

Projets sur financement : (EU) BREEDing Coffee for AgroForestry Systems

Auteurs et affiliations