Identification of QTLs conferring resistance to the genetic diversity of Ralstonia solanacearum in eggplant

Salgon Sylvia, Sauvage Christopher, Jourda Cyril, Daunay Marie-Christine, Wicker Emmanuel, Dintinger Jacques. 2016. Identification of QTLs conferring resistance to the genetic diversity of Ralstonia solanacearum in eggplant. In : Book of abstracts of the 6th International Bacterial Wilt Symposium. INRA. Toulouse : INRA, 40. International Bacterial Wilt Symposium. 6, Toulouse, France, 3 Juillet 2016/7 Juillet 2016.

Résumé : Eggplant (Solanum melongena) belongs to the large family of solanaceae comprising over 3000 species and including very important crops as tomato, potato and pepper. Eggplant is one of the most consumed vegetables in the world with a production reaching 49 million tons in 2013. Its production is mainly concentrated in tropical and subtropical areas. One of the principal limitation of eggplant production is the pathogen Ralstonia solanacearum, the causal agent of bacterial wilt. R. solanacearu represents a threat for crops due to its large host range, its persistence in fields and it extensive genotypic diversity. The species complex of R. solanacearum is divided into four monophyletic groups of strains. Whereas some plants species can be the host of only one phylotype, eggplant is a potential host for the four phylotypes. In such a situation, understanding the plant-pathogen interactions mechanisms will help developing cultivars with durable resistance to a broad range of R. solanacearum strains. For this purpose, an intraspecific population of 178 eggplant recombinant inbred lines (Rlls) was evaluated for resistance to R. solanacearum strains belongin to phylotypes I, IIA, 118, and Ill. In addition of 162 AFLP and SSR markers, genotyping-bysequencing (GBS) of the Rlls provided 661 SNPs used to construct a dense genetic map anchored to the physical map of tomato. Quantitative Trait Loci (QTL) analysis showed the presence of a major QTL (ERsl) specifically associated with resistance to phylotype I strains on the chromosome 9 of eggplant. Two broadspectrum QTLs were also identified on chromosome 2 and chromosome 5. Although less efficient than ERsl, these QTLs were found to partially control strains belonging to phylotype I, IIA and Ill. Molecular markers linked to QTLs will be very useful for breeding resistance to Ralstonia solanacearum in eggplant. The anchored map will also help to identify candidate genes underlying the so far identified resistance factors.

Auteurs et affiliations

• Salgon Sylvia, CIRAD-BIOS-UMR PVBMT (REU)
• Sauvage Christopher, INRA (FRA)
• Jourda Cyril, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-7799-3501
• Daunay Marie-Christine, INRA (REU)
• Wicker Emmanuel, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-0927-7404
• Dintinger Jacques, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/601568/)

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