Genetic diversity of 'Candidatus Phytoplasma palmicola' in Ghana and Mozambique

Pilet Fabian, Mendes Custódio Daniel, Yankey Egya Ndede, Lopes Parruque Manuel, Attivor Isaac Newton, Nkansah-Poku Joe, Vaz Antonia. 2022. Genetic diversity of 'Candidatus Phytoplasma palmicola' in Ghana and Mozambique. Phytopathogenic Mollicutes, 12 (1) : p. 69. TROPICSAFE Final Conference, San Giuliano, France, 3 Avril 2022/5 Avril 2022.

Url - éditeur : https://indianjournals.com/ijor.aspx?target=ijor:mollicutes&type=home

Résumé : For studying epidemiology and tracking 'Candidatus Phytoplasma palmicola' associated with the coconut lethal yellowing disease in Africa, a Multi Locus Sequence Typing (MLST) scheme was developed and applied to Ghanaian samples collected before 2010 across the country, and samples from the northern provinces of Cabo Delgado and Zambezia in Mozambique (Pilet et al., 2019). To estimate the capacity of the MLSA scheme developed to monitor the evolution in the composition of the pathogen population over time, and to confirm its capacity to detect emerging variants of the pathogen, the scheme was applied to a new set of samples. The sequences of the eight genes constituting the MLSA scheme were obtained for 102 Ghanaian DNA samples collected in 2017, and three Mozambican samples collected in 2018 in the province of Inhambane. In Ghana, two of the six sequence types (STs) identified were new ones. The ST2 is predominant in Central region, ST1 has become main type in the Western Region followed by the new emerging ST9. In Mozambique, the three samples collected in the Province of Inhambane represent three new STs. While apparently genetically linked to the STs from the province of Cabo Delgado and Zambezia, two samples show enough mutations to suggest an ancient introduction, possibly much older than its first observation in 2010 in Vilankulo, province of Inhambane. The third one (ST13) collected in the south of the province (Inharrime district) is very different from all other strains observed so far, suggesting a new and independent introduction of the pathogen. These data would be useful in devising policies on management measures against the disease. This study underlines the power of the MLSA scheme for monitoring 'Ca. P. palmicola'. It is necessary to continue both sampling and characterization of the pathogen in the various affected countries, and to develop similar tool for other diseases associated with phytoplasmas.

Mots-clés Agrovoc : maladie des plantes, phytoplasme, typage de séquence multilocus, génotype, génotypage

Mots-clés géographiques Agrovoc : Ghana, Mozambique

Mots-clés complémentaires : Jaunissement mortel, Candidatus Phytoplasma palmicola

Mots-clés libres : Coconut lethal yellowing disease, Multi locus sequence typing, Phytoplasmas

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Pilet Fabian, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0079-216X - auteur correspondant
• Mendes Custódio Daniel, Ministry of Agriculture and Food Security (MOZ)
• Yankey Egya Ndede, OPRI (GHA)
• Lopes Parruque Manuel, Provincial Directorate of Agriculture and Food Security (MOZ)
• Attivor Isaac Newton, OPRI (GHA)
• Nkansah-Poku Joe, OPRI (GHA)
• Vaz Antonia, Ministry of Agriculture and Food Security (MOZ)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/601633/)

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