In vitro tests for a rapid evaluation of antidiabetic potential of plant species containing caffeic acid derivatives: A validation by two well-known antidiabetic plants, Ocimum gratissimum L. leaf and Musanga cecropioides R. Br. ex Tedlie (Mu) Stem Bark

Awwad Abdulmomem, Poucheret Patrick, Idres Yanis A., Tshibangu Damien S. T., Servent Adrien, Ferrare Karine, Lazennec Françoise, Bidel Luc P.R., Cazals Guillaume, Tousch Didier. 2021. In vitro tests for a rapid evaluation of antidiabetic potential of plant species containing caffeic acid derivatives: A validation by two well-known antidiabetic plants, Ocimum gratissimum L. leaf and Musanga cecropioides R. Br. ex Tedlie (Mu) Stem Bark. Molecules, 26 (18):5566, 14 p.

https://doi.org/10.3390/molecules26185566

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
molecules-26-05566-v2.pdf
Télécharger (1MB) | Prévisualisation

Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : Plant bioactive extracts represent a major resource for identifying drugs and adjuvant therapy for type 2 diabetes. To promote early screening of plants' antidiabetic potential, we designed a four in vitro tests strategy to anticipate in vivo bioactivity. Two antidiabetic plants were studied: Ocimum gratissimum L. (Oc) leaf extract and Musanga cecropoides R. Br. ex Tedlie (Mu) stem bark extract. Chemical compositions were analyzed by LCMS and HPLC. Antidiabetic properties were measured based on (1) INS-1 cells for insulin secretion, (2) L6 myoblast cells for insulin sensitization (Glut-4 translocation), (3) L6 myoblast cells for protection against hydrogen peroxide (H2O2) oxidative stress (cell mortality), and (4) liver microsomial fraction for glucose-6-phosphastase activity (G6P). Oc extract increased insulin secretion and insulin sensitivity, whereas it decreased oxidative stress-induced cell mortality and G6P activity. Mu extract decreased insulin secretion and had no effect on insulin sensitivity or G6P activity, but it increased oxidative stress-induced cell mortality. Results were compared with NCRAE, an antidiabetic plant extract used as reference, previously characterized and reported with increased insulin secretion and insulin sensitivity, protection against oxidative stress, and decreased G6P activity. The proposed set of four in vitro tests combined with chemical analysis provided insight into the interest in rapid early screening of plant extract antidiabetic potential to anticipate pharmaco-toxicological in vivo effects.

Mots-clés Agrovoc : contrôle de maladies, diabète, plante médicinale, propriété physicochimique, Ocimum, feuille, écorce, expérimentation in vitro, insuline

Mots-clés géographiques Agrovoc : République démocratique du Congo

Mots-clés complémentaires : Ocimum gratissimum, Musanga cecropoides

Mots-clés libres : Ocimum gratissimum, Musanga cecropioides, In vitro antidiabetic, Insulin secretion, Glucose uptake, Protection cells against H2O2 oxidative stress, Caffeoyl derivatives

Classification Agris : S50 - Santé humaine

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations