MIRS measurement on cassava cell walls and flours. High-throughput phenotyping protocols (HTPP), WP3

Meghar Karima, Davrieux Fabrice, Alamu Emmanuel Oladeji. 2021. MIRS measurement on cassava cell walls and flours. High-throughput phenotyping protocols (HTPP), WP3. Montpellier : RTBfoods Project-CIRAD, 12 p.

Résumé : The purpose of this SOP is to use MIRS to predict the cooking behaviour of cassava from different varieties by using spectra of corresponding cassava cell walls or flour. For this purpose, eighty-nine samples of cell walls from 37 different varieties (2 or 3 roots per genotype) provided by CIAT (Colombia) were analysed by using Thermo Scientific™ Nicolet™ iS50R Research FTIR Spectrometers in the 4000-400 cm-1 region. For the repeatability test, 8 spectra of the same flour sample were acquired in order to determine the optimum number of MIRS measurements (replicates) required to be representative of the sample. The RMS obtained for 5 combinations of two replicates (2-3, 1-2, 3-5, 3-7 and 6-8) are 457, 1066, 710, 1352 and 614 μabs respectively. The RMS values between 2 replicates, randomly selected, were lower than the RMS mean for all the replicates. These results indicate that two replicates of the sample result to a spectral dispersion (variability) similar to the one obtained with 8 repetitions.

Mots-clés libres : MIRS, Cassava cell walls, Cooking behaviour

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

Auteurs et affiliations

• Meghar Karima, CIRAD-PERSYST-UMR Qualisud (FRA)

Contributeurs et affiliations

• Davrieux Fabrice, CIRAD-PERSYST-UMR Qualisud (REU) - collaborateur
• Alamu Emmanuel Oladeji, IITA (NGA) - collaborateur

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602132/)

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