Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti

De Groot Arjan, Dulermo Rémi, Ortet Philippe, Blanchard Laurence, Guérin Philippe, Fernandez Bernard, Vacherie Benoit, Dossat Carole, Jolivet Edmond, Siguier Patricia, Chandler Michael, Barakat Mohamed, Dedieu Alain, Barbe Valérie, Heulin Thierry, Sommer Suzanne, Achouak Wafa, Armengaud Jean. 2009. Alliance of proteomics and genomics to unravel the specificities of Sahara bacterium Deinococcus deserti. PLoS Genetics, 5 (3):e1000434, 12 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/CP001114

Quartile : Outlier, Sujet : GENETICS & HEREDITY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Résumé : To better understand adaptation to harsh conditions encountered in hot arid deserts, we report the first complete genome sequence and proteome analysis of a bacterium, Deinococcus deserti VCD115, isolated from Sahara surface sand. Its genome consists of a 2.8-Mb chromosome and three large plasmids of 324 kb, 314 kb, and 396 kb. Accurate primary genome annotation of its 3,455 genes was guided by extensive proteome shotgun analysis. From the large corpus of MS/MS spectra recorded, 1,348 proteins were uncovered and semiquantified by spectral counting. Among the highly detected proteins are several orphans and Deinococcus-specific proteins of unknown function. The alliance of proteomics and genomics high-throughput techniques allowed identification of 15 unpredicted genes and, surprisingly, reversal of incorrectly predicted orientation of 11 genes. Reversal of orientation of two Deinococcus-specific radiation-induced genes, ddrC and ddrH, and identification in D. deserti of supplementary genes involved in manganese import extend our knowledge of the radiotolerance toolbox of Deinococcaceae. Additional genes involved in nutrient import and in DNA repair (i.e., two extra recA, three translesion DNA polymerases, a photolyase) were also identified and found to be expressed under standard growth conditions, and, for these DNA repair genes, after exposure of the cells to UV. The supplementary nutrient import and DNA repair genes are likely important for survival and adaptation of D. deserti to its nutrient-poor, dry, and UV-exposed extreme environment.

Auteurs et affiliations

• De Groot Arjan, CEA (FRA) - auteur correspondant
• Dulermo Rémi, CEA (FRA)
• Ortet Philippe, CEA (FRA)
• Blanchard Laurence, CEA (FRA)
• Guérin Philippe, CEA (FRA)
• Fernandez Bernard, CEA (FRA)
• Vacherie Benoit, CEA (FRA)
• Dossat Carole, CEA (FRA)
• Jolivet Edmond, Université Paris-Sud (FRA)
• Siguier Patricia, CNRS (FRA)
• Chandler Michael, CNRS (FRA)
• Barakat Mohamed, CNRS (FRA)
• Dedieu Alain, CEA (FRA)
• Barbe Valérie, CEA (FRA)
• Heulin Thierry, CEA (FRA)
• Sommer Suzanne, Université Paris-Sud (FRA)
• Achouak Wafa, CNRS (FRA)
• Armengaud Jean, CEA (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602177/)

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