Is succeeding in superinfection a characteristic of the recombinant TYLCVIS76?

Urbino Cica, Jammes Margaux, Balland Julie, Blanc Stéphane, Peterschmitt Michel. 2022. Is succeeding in superinfection a characteristic of the recombinant TYLCVIS76?. . SFV. Sète : SFV, Résumé, 1 p. International Symposium on ssDNA Viruses, Sète, France, 26 Septembre 2022/1 Octobre 2022.

Résumé : TYLCV-IS76 is a natural recombinant of tomato yellow curl virus (TYLCV) in which 76 nts of the intergenic region were replaced by the homologous sequence of tomato yellow curl Sardinia virus (TYLCSV). TYLCV-IS76 emerged in Morocco and was shown to be highly competitive in resistant cultivar carrying the Ty-1 resistance gene, a gene that prevents symptoms and reduces viral load. In competition experiments, the accumulation level of TYLCV-IS76 was higher than that of parental viruses regardless of its time of infection, i.e., simultaneously with parental viruses or with 1 or 4 months delays. In addition to reveal the super fitness of this recombinant, this result suggests that cross protection, a mechanism that could have occurred between closely related genomes, does not protect resistant plants infected with TYLCV against TYLCV-IS76. As gene silencing-based antiviral defence is involved in the Ty-1 resistance mechanism and can also participate to cross protection, the question was: is the superinfection success a specific feature of TYLCV-IS76, or is it a more general feature of tomatoinfecting begomoviruses? This question was addressed experimentally by testing if viruses, slightly different from TYLCV-IS76, can also superinfect tomato plants. A Ty-1 resistant tomato cultivar and an isogenic susceptible one were used to test if the Ty-1 gene is involved in the superinfection phenomenon. This study may unveil a new aspect of the unusual fitness of TYLCV-IS76, and shed new light on potential interactions prevailing in plants co-infected with begomoviruses.

Auteurs et affiliations

• Urbino Cica, CIRAD-BIOS-UMR PHIM (FRA)
• Jammes Margaux, CIRAD-BIOS-UMR PHIM (FRA)
• Balland Julie, Université de Montpellier (FRA)
• Blanc Stéphane, INRAE (FRA)
• Peterschmitt Michel, CIRAD-BIOS-UMR PHIM (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/602932/)

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