Occurrence, serotypes and virulence characteristics of shiga-toxin-producing Escherichia coli isolates from goats on communal rangeland in South Africa

Malahlela Mogaugedi, Cenci-Goga Beniamino T., Marufu Munyaradzi Christopher, Fonkui Thierry Y., Grispoldi Luca, Etter Eric, Kalake Alan, Karama Musafiri. 2022. Occurrence, serotypes and virulence characteristics of shiga-toxin-producing Escherichia coli isolates from goats on communal rangeland in South Africa. Toxins, 14 (5):353, 14 p.

https://doi.org/10.3390/toxins14050353

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Résumé : Shiga-toxin-producing Escherichia coli is a foodborne pathogen commonly associated with human disease characterized by mild or bloody diarrhea hemorrhagic colitis and hemolytic uremic syndrome. This study investigated the occurrence of STEC in fecal samples of 289 goats in South Africa using microbiological culture and PCR. Furthermore, 628 goat STEC isolates were characterized by serotype (O:H) and major virulence factors by PCR. STEC was found in 80.2% (232/289) of goat fecal samples. Serotyping of 628 STEC isolates revealed 63 distinct serotypes including four of the major top seven STEC serogroups which were detected in 12.1% (35/289) of goats: O157:H7, 2.7% (8/289); O157:H8, 0.3%, (1/289); O157:H29, 0.3% (1/289); O103:H8, 7.6% (22/289); O103:H56, 0.3% (1/289); O26:H2, 0.3% (1/289); O111:H8, 0.3% (1/289) and 59 non-O157 STEC serotypes. Twenty-four of the sixty-three serotypes were previously associated with human disease. Virulence genes were distributed as follows: stx1, 60.6% (381/628); stx2, 72.7% (457/628); eaeA, 22.1% (139/628) and hlyA, 78.0% (490/628). Both stx1 and stx2 were found in 33.4% (210/628) of isolates. In conclusion, goats in South Africa are a reservoir and potential source of diverse STEC serotypes that are potentially virulent for humans. Further molecular characterization will be needed to fully assess the virulence potential of goat STEC isolates and their capacity to cause disease in humans.

Mots-clés Agrovoc : Escherichia coli

Mots-clés géographiques Agrovoc : Afrique du Sud

Mots-clés libres : Escherichia coli, Shiga-toxin-producing, Goats, South Africa

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : National Research Foundation, South African Medical Research Council

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