Detection of west nile virus lineage 2 in eastern romania and first identification of sindbis virus rna in mosquitoes analyzed using high-throughput microfluidic real-time PCR

Crivei Luciana Alexandra, Moutailler Sara, Gonzalez Gaëlle, Lowenski Steeve, Crivei Ioana Cristina, Porea Daniela, Anita Dragos Constantin, Ratoi Ioana Alexandra, Zientara Stéphan, Oslobanu Luanda Elena, Tomazotos Alexandru, Savuta Gheorghe, Lecollinet Sylvie. 2023. Detection of west nile virus lineage 2 in eastern romania and first identification of sindbis virus rna in mosquitoes analyzed using high-throughput microfluidic real-time PCR. Viruses, 15 (1), n.spéc State-of-the-Art Arbovirus Research in Europe 2022:186, 14 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/?term=OQ134778 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/?term=OQ134779

Résumé : The impact of mosquito-borne diseases on human and veterinary health is being exacerbated by rapid environmental changes caused mainly by changing climatic patterns and globalization. To gain insight into mosquito-borne virus circulation from two counties in eastern and southeastern Romania, we have used a combination of sampling methods in natural, urban and peri-urban sites. The presence of 37 mosquito-borne viruses in 16,827 pooled mosquitoes was analyzed using a high-throughput microfluidic real-time PCR assay. West Nile virus (WNV) was detected in 10/365 pools of Culex pipiens (n = 8), Culex modestus (n = 1) and Aedes vexans (n = 1) from both studied counties. We also report the first molecular detection of Sindbis virus (SINV) RNA in the country in one pool of Culex modestus. WNV infection was confirmed by real-time RT-PCR (10/10) and virus isolation on Vero or C6/36 cells (four samples). For the SINV-positive pool, no cytopathic effectwas observed after infection of Vero or C6/36 cells, but no amplification was obtained in conventional SINV RT-PCR. Phylogenetic analysis of WNV partial NS5 sequences revealed that WNV lineage 2 of the Central-Southeast European clade, has a wider circulation in Romania than previously known.

Mots-clés Agrovoc : virus du nil occidental, virus de sindbis, arbovirus, maladie transmise par vecteur, PCR, Culex pipiens, Aedes vexans

Mots-clés géographiques Agrovoc : Roumanie

Mots-clés complémentaires : Culex modestus, test PCR en temps réel

Mots-clés libres : Arbovirus, West Nile virus, Sindbis virus, Romania

Classification Agris : L73 - Maladies des animaux
S50 - Santé humaine
L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Crivei Luciana Alexandra, Iași University of Life Sciences (ROU) - auteur correspondant
• Moutailler Sara, ANSES (FRA)
• Gonzalez Gaëlle, ANSES (FRA)
• Lowenski Steeve, ANSES (FRA)
• Crivei Ioana Cristina, Iași University of Life Sciences (ROU)
• Porea Daniela, Iași University of Life Sciences (ROU)
• Anita Dragos Constantin, Iași University of Life Sciences (ROU)
• Ratoi Ioana Alexandra, Iași University of Life Sciences (ROU)
• Zientara Stéphan, AFSSA (FRA)
• Oslobanu Luanda Elena, Iași University of Life Sciences (ROU)
• Tomazotos Alexandru, Bernhard Nocht Institute for Tropical Medicine (DEU)
• Savuta Gheorghe, Iași University of Life Sciences (ROU)
• Lecollinet Sylvie, CIRAD-BIOS-UMR ASTRE (GLP) ORCID: 0000-0001-8535-2133

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603382/)

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