Genetic variation in host-specific competitiveness of the symbiont Rhizobium leguminosarum symbiovar viciae

Boivin Stéphane, Mahé Frédéric, Debellé Frédéric, Pervent Marjorie, Tancelin Mathilde, Tauzin Marc, Wielbo Jerzy, Mazurier Sylvie, Young Peter, Lepetit Marc. 2021. Genetic variation in host-specific competitiveness of the symbiont Rhizobium leguminosarum symbiovar viciae. Frontiers in Plant Science, 12:719987, 17 p.

https://doi.org/10.3389/fpls.2021.719987

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Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Legumes of the Fabeae tribe form nitrogen-fixing root nodules resulting from symbiotic interaction with the soil bacteria Rhizobium leguminosarum symbiovar viciae (Rlv). These bacteria are all potential symbionts of the Fabeae hosts but display variable partner choice when co-inoculated in mixture. Because partner choice and symbiotic nitrogen fixation mostly behave as genetically independent traits, the efficiency of symbiosis is often suboptimal when Fabeae legumes are exposed to natural Rlv populations present in soil. A core collection of 32 Rlv bacteria was constituted based on the genomic comparison of a collection of 121 genome sequences, representative of known worldwide diversity of Rlv. A variable part of the nodD gene sequence was used as a DNA barcode to discriminate and quantify each of the 32 bacteria in mixture. This core collection was co-inoculated on a panel of nine genetically diverse Pisum sativum, Vicia faba, and Lens culinaris genotypes. We estimated the relative Early Partner Choice (EPC) of the bacteria with the Fabeae hosts by DNA metabarcoding on the nodulated root systems. Comparative genomic analyses within the bacterial core collection identified molecular markers associated with host-dependent symbiotic partner choice. The results revealed emergent properties of rhizobial populations. They pave the way to identify genes related to important symbiotic traits operating at this level.

Mots-clés Agrovoc : bactérie fixatrice de l'azote, Rhizobium, Rhizobium leguminosarum, Fixation de l'azote, nodosité racinaire, Vicia faba, Pisum sativum, symbiote, variation génétique, symbiose, inoculation des racines, Lens culinaris, Bacteria

Mots-clés libres : Rhizobium leguminosarum symbiovar viciae, Competitiveness, Pea, Fababean, Lentil, Fabeae, DNA metabarcoding, Symbiosis

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA)Caractérisation du déterminisme génétique du choix du partenaire symbiotique pour une amélioration de la symbiose fixatrice d'azote chez le pois

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