High-frequency synthetic apomixis in hybrid rice

Vernet Aurore, Meynard Donaldo, Lian Qichao, Mieulet Delphine, Gibert Olivier, Bissah Matilda Ntowa, Rivallan Ronan, Autran Daphné, Leblanc Olivier, Meunier Anne Cecile, Frouin Julien, Taillebois James E., Shankle Kyle, Khanday Imtiyaz, Mercier Raphael, Sundaresan Venkatesan, Guiderdoni Emmanuel. 2022. High-frequency synthetic apomixis in hybrid rice. Nature Communications, 13:7963, 13 p.

Url - jeu de données - Entrepôt autre : https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-11931

Résumé : Introducing asexual reproduction through seeds – apomixis – into crop species could revolutionize agriculture by allowing F1 hybrids with enhanced yield and stability to be clonally propagated. Engineering synthetic apomixis has proven feasible in inbred rice through the inactivation of three genes (MiMe), which results in the conversion of meiosis into mitosis in a line ectopically expressing the BABYBOOM1 (BBM1) parthenogenetic trigger in egg cells. However, only 10–30% of the seeds are clonal. Here, we show that synthetic apomixis can be achieved in an F1 hybrid of rice by inducing MiMe mutations and egg cell expression of BBM1 in a single step. We generate hybrid plants that produce more than 95% of clonal seeds across multiple generations. Clonal apomictic plants maintain the phenotype of the F1 hybrid along successive generations. Our results demonstrate that there is no barrier to almost fully penetrant synthetic apomixis in an important crop species, rendering it compatible with use in agriculture.

Mots-clés Agrovoc : apomixie, multiplication végétative, méiose, Oryza sativa, rendement des cultures, expression des gènes

Mots-clés libres : Agricultural genetics, Molecular engineering in plants, Plant embryogenesis

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

• Vernet Aurore, CIRAD-BIOS-UMR AGAP (FRA)
• Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
• Lian Qichao, Max Planck Institute for Plant Breeding Research (DEU)
• Mieulet Delphine, CIRAD-BIOS-UMR DIADE (FRA) ORCID: 0000-0001-6220-0372
• Gibert Olivier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1447-9003
• Bissah Matilda Ntowa, CSIR (GHA)
• Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
• Autran Daphné, Université de Montpellier (FRA)
• Leblanc Olivier, Université de Montpellier (FRA)
• Meunier Anne Cecile, CIRAD-BIOS-UMR AGAP (FRA)
• Frouin Julien, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0003-1591-0755
• Taillebois James E.
• Shankle Kyle, UC (USA)
• Khanday Imtiyaz, UC (USA) - auteur correspondant
• Mercier Raphael, Max Planck Institute for Plant Breeding Research (DEU) - auteur correspondant
• Sundaresan Venkatesan, UC (USA) - auteur correspondant
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603586/)

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