MtEFD and MtEFD2: Two transcription factors with distinct neofunctionalization in symbiotic nodule development

Jardinaud Marie-Françoise, Fromentin Justine, Auriac Marie-Christine, Moreau Sandra, Pecrix Yann, Taconnat Ludivine, Cottret Ludovic, Aubert Grégoire, Balzergue Sandrine, Burstin Judith, Carrère Sébastien, Gamas Pascal. 2022. MtEFD and MtEFD2: Two transcription factors with distinct neofunctionalization in symbiotic nodule development. Plant Physiology, 189 (3) : 1587-1607.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE138899

Résumé : Rhizobium–legume nitrogen-fixing symbiosis involves the formation of a specific organ, the root nodule, which provides bacteria with the proper cellular environment for atmospheric nitrogen fixation. Coordinated differentiation of plant and bacterial cells is an essential step of nodule development, for which few transcriptional regulators have been characterized. Medicago truncatula ETHYLENE RESPONSE FACTOR REQUIRED FOR NODULE DIFFERENTIATION (MtEFD) encodes an APETALA2/ETHYLENE RESPONSIVE FACTOR (ERF) transcription factor, the mutation of which leads to both hypernodulation and severe defects in nodule development. MtEFD positively controls a negative regulator of cytokinin signaling, the RESPONSE REGULATOR 4 (MtRR4) gene. Here we showed that that the Mtefd-1 mutation affects both plant and bacterial endoreduplication in nodules, as well as the expression of hundreds of genes in young and mature nodules, upstream of known regulators of symbiotic differentiation. MtRR4 expressed with the MtEFD promoter complemented Mtefd-1 hypernodulation but not the nodule differentiation phenotype. Unexpectedly, a nonlegume homolog of MtEFD, AtERF003 in Arabidopsis (Arabidopsis thaliana), could efficiently complement both phenotypes of Mtefd-1, in contrast to the MtEFD paralog MtEFD2 expressed in the root and nodule meristematic zone. A domain swap experiment showed that MtEFD2 differs from MtEFD by its C-terminal fraction outside the DNA binding domain. Furthermore, clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Cas9) mutagenesis of MtEFD2 led to a reduction in the number of nodules formed in Mtefd-1, with downregulation of a set of genes, including notably NUCLEAR FACTOR-YA1 (MtNF-YA1) and MtNF-YB16, which are essential for nodule meristem establishment. We, therefore, conclude that nitrogen-fixing symbiosis recruited two proteins originally expressed in roots, MtEFD and MtEFD2, with distinct functions and neofunctionalization processes for each of them.

Mots-clés Agrovoc : bactérie fixatrice de l'azote, Fixation de l'azote, Rhizobium, nodosité racinaire, symbiose, formation de nodosités, expression des gènes, formation atmosphérique, méristème

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Génomique comparative du cycle de l'azote entre M. truncatula et le pois : étude des potentialités de transfert des connaissances entre espèces modèles et cultivées

Auteurs et affiliations

• Jardinaud Marie-Françoise, Université de Toulouse (FRA)
• Fromentin Justine, Université de Toulouse (FRA)
• Auriac Marie-Christine, Université de Toulouse (FRA)
• Moreau Sandra, Université de Toulouse (FRA)
• Pecrix Yann, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-6537-3145
• Taconnat Ludivine, Université Paris-Saclay (FRA)
• Cottret Ludovic, Université de Toulouse (FRA)
• Aubert Grégoire, INRAE (FRA)
• Balzergue Sandrine, Université Paris-Saclay (FRA)
• Burstin Judith, INRAE (FRA)
• Carrère Sébastien, Université de Toulouse (FRA)
• Gamas Pascal, Université de Toulouse (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603830/)

Métriques

[ Page générée et mise en cache le 2024-12-18 ]