First Vanilla planifolia high-density genetic linkage map provides quantitative trait loci for resistance to Fusarium oxysporum

Favre Félicien, Jourda Cyril, Grisoni Michel, Chiroleu Frédéric, Dijoux Jean-Bernard, Jade Katia, Rivallan Ronan, Besse Pascale, Charron Carine. 2023. First Vanilla planifolia high-density genetic linkage map provides quantitative trait loci for resistance to Fusarium oxysporum. Plant Disease, 107 (10) : 2997-3006.

Résumé : Fusarium oxysporum f. sp. radicis-vanillae (Forv), the causal agent of root and stem rot disease, is the main pathogen affecting vanilla production. Sources of resistance have been reported in Vanilla planifolia G. Jackson ex Andrews, the main cultivated vanilla species. In this study, we developed the first high-density genetic map in this species with 1,804 genotyping-by-sequencing (GBS)-generated single nucleotide polymorphism (SNP) markers using 125 selfed progenies of the CR0040 traditional vanilla cultivar. Sixteen linkage groups (LG) were successfully constructed, with a mean of 113 SNPs and an average length of 207 cM per LG. The map had a high density with an average of 5.45 SNP every 10 cM and an average distance of 1.85 cM between adjacent markers. The first three LG were aligned against the first assembled chromosome of CR0040, and the other 13 LG were correctly associated with the other 13 assembled chromosomes. The population was challenged with the highly pathogenic Forv strain Fo072 using the root-dip inoculation method. Five traits were mapped, and 20 QTLs were associated with resistance to Fo072. Among the genes retrieved in the CR0040 physical regions associated with QTLs, genes potentially involved in biotic resistance mechanisms, coding for kinases, E3 ubiquitin ligases, pentatricopeptide repeat-containing proteins, and one leucine-rich repeat receptor underlying the qFo72_08.1 QTL have been highlighted. This study should provide useful resources for marker-assisted selection in V. planifolia.

Mots-clés Agrovoc : Vanilla planifolia, locus des caractères quantitatifs, carte génétique, résistance génétique, Fusarium oxysporum, polymorphisme génétique, pourriture des racines, marqueur génétique, résistance aux maladies, polymorphisme à nucléotide unique, Fusarium

Mots-clés géographiques Agrovoc : France, La Réunion

Mots-clés libres : Vanilla planifolia G. Jackson ex Andrews, Fusarium oxysporum f. sp. radicis-vanillae, Genotyping-by-Sequencing, High-density genetic map, Quantitative trait locus

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Regional Development Fund

Agences de financement hors UE : Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Université de La Réunion, Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche, Conseil Régional de La Réunion, Conseil Général de La Réunion

Auteurs et affiliations

• Favre Félicien, Université de la Réunion (REU)
• Jourda Cyril, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-7799-3501
• Grisoni Michel, CIRAD-BIOS-UMR PVBMT (MDG) ORCID: 0000-0001-9867-9098
• Chiroleu Frédéric, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-4874-5357
• Dijoux Jean-Bernard, CIRAD-BIOS-UMR PVBMT (REU)
• Jade Katia, CIRAD-BIOS-UMR PVBMT (REU)
• Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
• Besse Pascale, Université de la Réunion (REU)
• Charron Carine, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0003-0281-9936 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604071/)

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