Population structure of Pyricularia oryzae on rice in Vietnam reveals diversified populations with four pandemic and two endemic clusters.

Le Lieu Thi, Adreit Henri, Ha Loan Thi, Milazzo Joëlle, Lebrun Michel, Tharreau Didier, Pham Xuan Hoi, Nguyen Hai Thy Thanh, Fournier Elisabeth, Hoang Giang Thi. 2023. Population structure of Pyricularia oryzae on rice in Vietnam reveals diversified populations with four pandemic and two endemic clusters.. Fungal Genetics and Biology, 166:103794, 10 p.

Url - jeu de données - Entrepôt autre : https://doi.org/10.5061/dryad.dv41ns236

Résumé : We characterized the genetic structure of 609 strains of Pyricularia oryzae, the fungal pathogen causing rice blast disease, in three main regions in Vietnam using microsatellites (SSR) markers. From the 447 distinct multilocus genotypes identified, six genetic clusters were defined, all of them showing elevated genetic and genotypic diversities. Four of these clusters were related to rice-attacking lineages already described at the worldwide scale, whereas the two remaining clusters were endemic to Vietnam. Strains were unevenly distributed into the six clusters depending on their groups of rice variety (indica / japonica) or type of varieties (traditional / modern) of origin, but none of the clusters was specifically related to these two factors. The highest diversity of blast population was found in Northern mountainous area, and the lowest in Red River Delta in both terms of genetic diversity and gene diversity. Hierarchical AMOVAs confirmed that all three factors considered (rice variety group, type of variety origin and geography) significantly contributed to the population structure of P. oryzae in Vietnam, with highest contribution from rice variety group. Mating types were unevenly distributed among clusters. Combined with results of female fertility and linkage disequilibirum, we hypothesized that clonal reproduction probably occurred in all clusters, but that sexual reproduction likely took place at least in some restricted areas in the Northern mountainous area for strains belonging to the cluster related to the previously described recombinant lineage (worldwide lineage 1). Our study pictures the genetic diversity, population structure and reproductive mode of the blast fungus in central and north Vietnam, and shows that the observed population structure is explained by several factors, the most important one being the variability of rice variety. All these new information might help for elaborating appropriate strategies to controlling the blast disease.

Mots-clés Agrovoc : maladie des plantes, microsatellite, pyriculariose du riz, Pyricularia oryzae, variation génétique, génotype, Oryza sativa

Mots-clés géographiques Agrovoc : Viet Nam

Mots-clés libres : Pyricularia oryzae, Rice blast, SSR markers, Genetic diversity, Population structure, Vietnam

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Ambassade de France au Vietnam, Consortium of International Agricultural Research Centers, Agence Nationale de la Recherche

Projets sur financement : (FRA) Mediterranean Center for Environment and Biodiversity

Auteurs et affiliations

• Le Lieu Thi, Agricultural Genetics Institute (VNM)
• Adreit Henri, CIRAD-BIOS-UMR PHIM (FRA)
• Ha Loan Thi, Agricultural Genetics Institute (VNM)
• Milazzo Joëlle, Université de Montpellier (FRA)
• Lebrun Michel, USTH (VNM)
• Tharreau Didier, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0003-3961-6120
• Pham Xuan Hoi, Agricultural Genetics Institute (VNM)
• Nguyen Hai Thy Thanh, NOMAFSI (VNM)
• Fournier Elisabeth, INRAE (FRA) - auteur correspondant
• Hoang Giang Thi, Agricultural Genetics Institute (VNM) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604370/)

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