Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control

Lutrat Célia, Burckbuchler Myriam, Proveti Olmo Roenick, Beugnon Rémy, Fontaine Albin, Akbari Omar S., Argilés-Herrero Rafael, Baldet Thierry, Bouyer Jérémy, Marois Eric. 2023. Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control. Communications Biology, 6:646, 12 p.

https://doi.org/10.1038/s42003-023-05030-7

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Url - jeu de données - Entrepôt autre : https://doi.org/10.5281/zenodo.8032932

Résumé : Chemical control of disease vectoring mosquitoes Aedes albopictus and Aedes aegypti is costly, unsustainable, and increasingly ineffective due to the spread of insecticide resistance. The Sterile Insect Technique is a valuable alternative but is limited by slow, error-prone, and wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each Aedes species) based on fluorescence markers linked to the m and M sex loci, allowing for the isolation of transgenic males. Furthermore, we demonstrate how combining these sexing strains enables the production of non-transgenic males. In a mass-rearing facility, 100,000 first instar male larvae could be sorted in under 1.5 h with an estimated 0.01–0.1% female contamination on a single machine. Cost-efficiency analyses revealed that using these strains could result in important savings while setting up and running a mass-rearing facility. Altogether, these Genetic Sexing Strains should enable a major upscaling in control programmes against these important vectors.

Mots-clés Agrovoc : lutte biologique, vecteur de maladie, lutte anti-insecte, lâcher d'insectes stériles, génétique animale, animal transgénique, Aedes albopictus, Aedes aegypti

Mots-clés libres : CRISPR-Cas systems, Entomology, Genetic engineering

Classification Agris : L72 - Organismes nuisibles des animaux
L10 - Génétique et amélioration des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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