Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France

Cicculli Vincent, Ayhan Nazli, Luciani Léa, Pezzi Laura, Maitre Apolline, Decarreaux Dorine, De Lamballerie Xavier, Paoli Jean-Christophe, Vial Laurence, Charrel Rémi, Falchi Alessandra. 2022. Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France. Veterinary Medicine and Science, 8 (2) : 907-916.

https://doi.org/10.1002/vms3.700

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Résumé : Background: Several viruses belonging to the family Poxviridae can cause infections in humans and animals. In Corsica, livestock farming (sheep, goats, pigs, and cattle) is mainly mixed, leading to important interactions between livestock, wildlife, and human populations. This could facilitate the circulation of zoonotic diseases, and makes Corsica a good example for studies of tick-borne diseases. Objectives: To gain understanding on the circulation of poxviruses in Corsica, we investigated their presence in tick species collected from cattle, sheep, horses, and wild boar, and characterized them through molecular techniques. Methods: Ticks were tested using specific primers targeting conserved regions of sequences corresponding to two genera: parapoxvirus and orthopoxvirus. Results: A total of 3555 ticks were collected from 1549 different animals (687 cattle, 538 horses, 106 sheep, and 218 wild boars). They were tested for the presence of parapoxvirus DNA on one hand and orthopoxvirus DNA on the other hand using Pangeneric real-time TaqMan assays. Orthopoxvirus DNA was detected in none of the 3555 ticks. Parapoxvirus DNA was detected in 6.6% (36/544) of ticks collected from 23 cows from 20 farms. The remaining 3011 ticks collected from horses, wild boars, and sheep were negative. The infection rate in cow ticks was 8.0% (12/148) in 2018 and 6.0% (24/396) in 2019 (p = 0.57). Parapoxvirus DNA was detected in 8.5% (5/59) of Hyalomma scupense pools, 8.2% (15/183) of Hyalomma marginatum pools, and 6.7% (16/240) of Rhipicephalus bursa pools (p = 0.73). We successfully amplified and sequenced 19.4% (7/36) of the positive samples which all corresponded to pseudocowpox virus. Conclusions: Obviously, further studies are needed to investigate the zoonotic potential of pseudocowpox virus and its importance for animals and public health.

Mots-clés Agrovoc : Ixodidae, Parapoxvirus, animal sauvage, maladie transmissible par tiques, bovin, transmission des maladies, vecteur de maladie, zoonose, identification, ovin, bétail, taxonomie, biologie moléculaire