A natural single nucleotide mutation in the small RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria

Brual Typhaine, Effantin Geraldine, Baltenneck Julie, Attaiech Laetitia, Grobois Cloé, Royer Monique, Cigna Jérémy, Faure Denis, Hugouvieux-Cotte-Pattat Nicole, Gueguen Erwan. 2023. A natural single nucleotide mutation in the small RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria. In : 16th Plant Bacteria Meeting. Abstract Booklet. INRAE. Aussois : INRAE, Résumé, 41. Rencontres plantes-bactéries. 16, Aussois, France, 20 Mars 2023/24 Mars 2023.

Résumé : The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase gene clusters. Analogy with genes described in other bacteria suggests that the clusters ooc and zms are involved in the production of secondary metabolites of the oocydin and zeamine families, respectively. A third cluster that we named ssm for solani secondary metabolite had an unknown function. In this study, we constructed mutants impaired in each of the three secondary metabolite clusters ssm, ooc, and zms to compare first the phenotype of the D. solani wild-type strain D s0432-1 with its associated mutants. We demonstrated the antimicrobial functions of these three PKS/NRPS clusters against bacteria, yeasts or fungi. The secondary metabolite cluster ssm, conserved in several other Dickeya species, produces a secondary metabolite inhibiting yeasts. Phenotyping and comparative genomics of different D. solani wild-type isolates revealed that the small regulatory RNA ArcZ plays a major role in the control of the clusters ssm and zms. A single-point mutation, conserved in some Dickeya wild-type strains, including the type strain IPO 2222, impairs the ArcZ function by affecting its processing into an active form.

Auteurs et affiliations

• Brual Typhaine, Université de Lyon (FRA)
• Effantin Geraldine, CNRS (FRA)
• Baltenneck Julie, Université de Lyon (FRA)
• Attaiech Laetitia, CIRI (FRA)
• Grobois Cloé, INSA de Lyon (FRA)
• Royer Monique, CIRAD-BIOS-UMR PHIM (FRA)
• Cigna Jérémy, French Federation of Seed Potato Growers (FRA)
• Faure Denis, CNRS (FRA)
• Hugouvieux-Cotte-Pattat Nicole, INSA de Lyon (FRA)
• Gueguen Erwan, INSA de Lyon (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/606506/)

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