Assessment of virus and Leptospira carriage in bats in France

Arnaout Youssef, Picard-Meyer Evelyne, Robardet Emmanuelle, Cappelle Julien, Cliquet Florence, Touzalin Frédéric, Jimenez Giacomo, Djelouadji Zouheira. 2023. Assessment of virus and Leptospira carriage in bats in France. PloS One, 18 (10):e0292840, 21 p.

Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/MZ893192 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/MZ893219

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Résumé : With over 1,400 species worldwide, bats represent the second largest order of mammals after rodents, and are known to host major zoonotic pathogens. Here, we estimate the presence of pathogens in autochthonous bat populations. First, we set out to check our samples for PCR amplification efficiency by assessing the occurrence of inhibited PCR reactions from different types of bat samples with amplifying the housekeeping gene β-actin. Second, we investigated the presence of five targeted pathogens in a French bat population using PCR. We targeted viral RNA of Canine distemper virus, Alphacoronavirus, Lyssavirus, Rotavirus and bacterial Leptospira DNA. To do so, we screened for these viruses in bat faecal samples as well as in oropharyngeal swab samples. The presence of Leptospira was assessed in urine, kidney, lung and faecal samples. Results showed a frequency of inhibited reactions ranging from 5 to 60% of samples, varying according to the sample itself and also suspected to vary according to sampling method and the storage buffer solution used, demonstrating the importance of the sampling and storage on the probability of obtaining negative PCR results. For pathogen assessment, rotavirus and alphacoronavirus RNA were detected in Myotis myotis, Myotis daubentonii, Myotis emarginatus and Rhinolophus ferrumequinum bats. Rotaviruses were also detected in Barbastella barbastellus. The presence of alphacoronavirus also varied seasonally, with higher frequencies in late summer and October, suggesting that juveniles potentially play an important role in the dynamics of these viruses. Leptospira DNA was detected in M. myotis and M. daubentonii colonies. The 16S rRNA sequences obtained from Leptospira positive samples showed 100% genetic identity with L. borgpetersenii. Neither canine distemper virus nor lyssavirus RNA were detected in any of the tested samples. This study is the first to show the presence of Leptospira in autochthonous French bats in addition to coronavirus and rotavirus RNA previously reported in European autochthonous bats.

Mots-clés Agrovoc : transmission des maladies, virus des animaux, Lyssavirus, Alphacoronavirus, Leptospira, maladie bactérienne, virus maladie de carré, rotavirus

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Alphacoronavirus, Α-CoV, CDV, Canine distemper virus, DNA, Deoxyribonucleic acid, PCR, Polymerase chain reaction, RdRp, RNA-dependent RNA polymerase, RNA, Ribonucleic acid

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Agences de financement hors UE : VetAgro Sup

Programme de financement européen : H2020

Projets sur financement : (EU) Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards

Auteurs et affiliations

• Arnaout Youssef, ANSES (FRA)
• Picard-Meyer Evelyne, ANSES (FRA)
• Robardet Emmanuelle, ANSES (FRA)
• Cappelle Julien, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0001-7668-1971
• Cliquet Florence, ANSES (FRA)
• Touzalin Frédéric, University college Dublin (IRL)
• Jimenez Giacomo, CPEPESC (FRA)
• Djelouadji Zouheira, INRAE (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/606745/)

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