Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage

El Hamss Hajar, Maruthi M.N., Omongo Christopher A., Wang Hua-Ling, van Brunschot Sharon, Colvin John, Delatte Hélène. 2023. Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage. Microbiological Research, 278:127538, 10 p.

https://doi.org/10.1016/j.micres.2023.127538

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : Within the Bemisia tabaci group of cryptic whitefly species, many are damaging agricultural pests and plant-virus vectors, conferring upon this group the status of one of the world's top 100 most invasive and destructive species, affecting farmers' income and threatening their livelihoods. Studies on the microbiome of whitefly life stages are scarce, although their composition and diversity greatly influence whitefly fitness and development. We used high-throughput sequencing to understand microbiome diversity in different developmental stages of the B. tabaci sub-Saharan Africa 1 (SSA1-SG1) species of the whitefly from Uganda. Endosymbionts (Portiera, Arsenophonus, Wolbachia, and Hemipteriphilus were detected but excluded from further statistical analysis as they were not influenced by life stage using Permutational Multivariate Analysis of Variance Using Distance Matrices (ADONIS, p = 0.925 and Bray, p = 0.903). Our results showed significant differences in the meta microbiome composition in different life stages of SSA1-SG1. The diversity was significantly higher in eggs (Shannon, p = 0.024; Simpson, p = 0.047) than that in nymphs and pupae, while the number of microbial species observed by the amplicon sequence variant (ASV) was not significant (n(ASV), p = 0.094). At the phylum and genus levels, the dominant constituents in the microbiome changed significantly during various developmental stages, with Halomonas being present in eggs, whereas Bacillus and Caldalkalibacillus were consistently found across all life stages. These findings provide the first description of differing meta microbiome diversity in the life stage of whiteflies, suggesting their putative role in whitefly development.

Mots-clés Agrovoc : Bemisia tabaci, vecteur de maladie, stade de développement, méthode statistique

Mots-clés géographiques Agrovoc : Ouganda, Afrique au sud du Sahara, Afrique

Mots-clés libres : SSA1-SG1, Life stage, Meta microbiome, Illumina, 16 S rDNA gene

Classification Agris : H10 - Ravageurs des plantes
F40 - Écologie végétale

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Bill and Melinda Gates Foundation

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