INDICANTS project : INnovative DIagnostiCs for bANana paThogens Surveillance

Robène Isabelle, Bothma Sheryl, Maillot-Lebon Véronique, Pecrix Yann, Fenelon Babbitha, Matthews Megan, Reynaud Bernard, Coetzee Beatrix, Arietta Salgado Mikel, Wicker Emmanuel, Chilin-Charles Yolande, Gianinazzi Camilo, Mostert Diane, Viljoen Altus. 2023. INDICANTS project : INnovative DIagnostiCs for bANana paThogens Surveillance. In : Southern African Society for Plant Pathologists Symposium (SASPP): Abstracts. SASPP. Stellenbosch : SASPP, Résumé, p. 11. Southern African Society for Plant Pathologists Symposium (SASPP), Stellenbosch, Afrique du Sud, 3 Avril 2023/3 Avril 2023.

Résumé : Banana is a major staple in developing countries and the most‐eaten fruit in Europe. Global banana production is constrained by several diseases responsible for yield losses and low productivity which may compromise food security. Four vascular diseases are of particular concern: Fusarium wilt disease of banana caused by the fungus Fusarium oxysporum f.sp. cubense (Foc TR4), Moko and banana blood diseases, caused by Ralstonia solanacearum and R. syzygii subsp. celebesensis, respectively and Xanthomonas wilt of banana caused by Xanthomonas vasicola pv. musacearum. Efficient surveillance and plant disease management require the availability of Point of care (POC) diagnostics that can be operated directly on‐site. The main objectives of the INDICANTS project were to (i) develop low‐cost multi‐pathogens LAMP (Loop‐mediated isothermal amplification) assays that will be further processed into ready‐to‐use kits by a private company; (ii) compare different simplified DNA extraction methods for field application; (iii) validate the LAMP protocols via inter laboratory tests and field surveys. Specific DNA targets were selected for the bacterial pathogens using in silico compara tive genomic analysis of target and non‐target genomes. LAMP primer sets were designed for each pathogen and showed 100% specificity when assayed on a wide range of target and non‐target strains. A limit of detection of 104 CFU/ml was obtained for the LAMP assays. A simplified DNA extraction method from banana tissue was developed and successfully validated in a banana plantation infested with FocTR4 using several candidate LAMP primer sets. Ready‐to‐use kits based on these protocols are currently being developed by a private company. These POC rapid diagnostic tools will allow rapid identification of the different diseases in the field and improve disease management.

Auteurs et affiliations

• Robène Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
• Bothma Sheryl, Stellenbosch University (ZAF)
• Maillot-Lebon Véronique, CIRAD-BIOS-UMR PVBMT (REU)
• Pecrix Yann, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-6537-3145
• Fenelon Babbitha, CIRAD-BIOS-UMR PVBMT (REU)
• Matthews Megan, CIRAD-BIOS-UMR PHIM (FRA)
• Reynaud Bernard
• Coetzee Beatrix, Stellenbosch University (ZAF)
• Arietta Salgado Mikel
• Wicker Emmanuel, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0003-0927-7404
• Chilin-Charles Yolande, CIRAD-BIOS-UMR PHIM (GLP)
• Gianinazzi Camilo, Qualiplante (FRA)
• Mostert Diane, Stellenbosch University (ZAF)
• Viljoen Altus, Stellenbosch University (ZAF)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/607862/)

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