Mapping of QTLs associated with biological nitrogen fixation traits in peanuts (Arachis hypogaea l.) using an interspecific population derived from the cross between the cultivated species and its wild ancestors

Nzepang Darius T., Gully Djamel, Nguepjop Joël Romaric, Zaiya Zazou Arlette, Tossim Hodo-Abalo, Sambou Aissatou, Rami Jean-François, Hocher Valérie, Fall Saliou, Svistoonoff Sergio, Fonceka Daniel. 2023. Mapping of QTLs associated with biological nitrogen fixation traits in peanuts (Arachis hypogaea l.) using an interspecific population derived from the cross between the cultivated species and its wild ancestors. Genes, 14 (4), n.spéc. Peanut Genetics and Omics:797, 20 p.

https://doi.org/10.3390/genes14040797

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
genes-14-00797-v2.pdf
Télécharger (2MB) | Prévisualisation

Résumé : Peanuts (Arachis hypogaea L.) are an allotetraploid grain legume mainly cultivated by poor farmers in Africa, in degraded soil and with low input systems. Further understanding nodulation genetic mechanisms could be a relevant option to facilitate the improvement of yield and lift up soil without synthetic fertilizers. We used a subset of 83 chromosome segment substitution lines (CSSLs) derived from the cross between a wild synthetic tetraploid AiAd (Arachis ipaensis × Arachis duranensis)4× and the cultivated variety Fleur11, and evaluated them for traits related to BNF under shade-house conditions. Three treatments were tested: without nitrogen; with nitrogen; and without nitrogen, but with added0 Bradyrhizobium vignae strain ISRA400. The leaf chlorophyll content and total biomass were used as surrogate traits for BNF. We found significant variations for both traits specially linked to BNF, and four QTLs (quantitative trait loci) were consistently mapped. At all QTLs, the wild alleles decreased the value of the trait, indicating a negative effect on BNF. A detailed characterization of the lines carrying those QTLs in controlled conditions showed that the QTLs affected the nitrogen fixation efficiency, nodule colonization, and development. Our results provide new insights into peanut nodulation mechanisms and could be used to target BNF traits in peanut breeding programs.

Mots-clés Agrovoc : Arachis hypogaea, locus des caractères quantitatifs, Fixation de l'azote, Fixation biologique de l'azote, Bradyrhizobium

Mots-clés complémentaires : Bradyrhizobium vignae

Mots-clés libres : Peanut, Symbiosis, Wild species, Sustainable agriculture

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations