The causal mutation leading to sweetness in modern white lupin cultivars

Mancinotti Davide, Czepiel Katarzyna, Taylor Jemma, Golshadi Galehshahi Hajar, Moller Lillian A., Jensen Mikkel K., Motawia Mohammed Saddik, Hufnagel Maciel Bárbara, Soriano Alexandre, Yeheyis Likawent, Kjaerulff Louise, Péret Benjamin, Staerk Dan, Wendt Toni, Nelson Matthew, Kroc Magdalena, Geu-Flores Fernando. 2023. The causal mutation leading to sweetness in modern white lupin cultivars. Science Advances, 9 (31):eadg8866, 10 p.

https://dx.doi.org/10.1126/sciadv.adg8866

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Résumé : Lupins are high-protein crops that are rapidly gaining interest as hardy alternatives to soybean; however, they accumulate antinutritional alkaloids of the quinolizidine type (QAs). Lupin domestication was enabled by the discovery of genetic loci conferring low QA levels (sweetness), but the precise identity of the underlying genes remains uncertain. We show that pauper, the most common sweet locus in white lupin, encodes an acetyltransferase (AT) unexpectedly involved in the early QA pathway. In pauper plants, a single-nucleotide polymorphism (SNP) strongly impairs AT activity, causing pathway blockage. We corroborate our hypothesis by replicating the pauper chemotype in narrow-leafed lupin via mutagenesis. Our work adds a new dimension to QA biosynthesis and establishes the identity of a lupin sweet gene for the first time, thus facilitating lupin breeding and enabling domestication of other QA-containing legumes.

Mots-clés Agrovoc : biosynthèse, mutation, rotation culturale

Classification Agris : F08 - Systèmes et modes de culture

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement européennes : European Research Council, European Commission

Agences de financement hors UE : Villum Fonden, Novo Nordisk Fonden, Ministry of Agriculture and Rural Development

Programme de financement européen : H2020

Projets sur financement : (EU) Unravelling cluster root development in white lupin

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