The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner

Xiao Gui, Laksanavilat Nutthalak, Cesari Stella, Lambou Karine, Baudin Maël, Jalilian Ahmad, Telebanco-Yanoria Mary Jeanie, Chalvon Véronique, Meusnier Isabelle, Fournier Elisabeth, Tharreau Didier, Zhou Bo, Wu Jun, Kroj Thomas. 2024. The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner. Nature Plants, 10 : 994-1004.

Résumé : Blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating rice diseases. Disease resistance genes such as Pi-ta or Pi-ta2 are critical in protecting rice production from blast. Published work reports that Pi-ta codes for a nucleotide-binding and leucine-rich repeat domain protein (NLR) that recognizes the fungal protease-like effector AVR-Pita by direct binding. However, this model was challenged by the recent discovery that Pi-ta2 resistance, which also relies on AVR-Pita detection, is conferred by the unconventional resistance gene Ptr, which codes for a membrane protein with a cytoplasmic armadillo repeat domain. Here, using NLR Pi-ta and Ptr RNAi knockdown and CRISPR/Cas9 knockout mutant rice lines, we found that AVR-Pita recognition relies solely on Ptr and that the NLR Pi-ta has no role in it, indicating that it is not the Pi-ta resistance gene. Different alleles of Ptr confer different recognition specificities. The A allele of Ptr (PtrA) detects all natural sequence variants of the effector and confers Pi-ta2 resistance, while the B allele of Ptr (PtrB) recognizes a restricted set of AVR-Pita alleles and, thereby, confers Pi-ta resistance. Analysis of the natural diversity in AVR-Pita and of mutant and transgenic strains identified one specific polymorphism in the effector sequence that controls escape from PtrB-mediated resistance. Taken together, our work establishes that the M. oryzae effector AVR-Pita is detected in an allele-specific manner by the unconventional rice resistance protein Ptr and that the NLR Pi-ta has no function in Pi-ta resistance and the recognition of AVR-Pita.

Mots-clés Agrovoc : résistance aux maladies, Oryza sativa, résistance génétique, effecteur moléculaire, Magnaporthe, relation hôte pathogène, amélioration des plantes

Mots-clés complémentaires : Magnaporthe oryzae

Mots-clés libres : Plant genetics, Plant immunity, Plant molecular biology

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Agences de financement hors UE : Agence Nationale de la Recherche, Swiss National Science Foundation

Projets sur financement : (FRA) Récepteurs immunitaires de type NLR pour des résistances plus durables chez les céréales, (FRA) Reconnaissance de ligands et activation de l'immunité par des paires de récepteurs NLRs chez les plantes, (EU) Virulence Activities of the Metalloprotease Effector AVR-Pita from the rice blast fungus Magnaporthe oryzae

Auteurs et affiliations

• Xiao Gui, IRRI [International Rice Research Institute] (PHL)
• Laksanavilat Nutthalak, Université de Montpellier (FRA)
• Cesari Stella, INRAE (FRA)
• Lambou Karine, Université de Montpellier (FRA)
• Baudin Maël, Université d'Angers (FRA)
• Jalilian Ahmad, Ferdowsi University of Mashhad (IRN)
• Telebanco-Yanoria Mary Jeanie, IRRI [International Rice Research Institute] (PHL)
• Chalvon Véronique, INRAE (FRA)
• Meusnier Isabelle, INRAE (FRA)
• Fournier Elisabeth, INRAE (FRA)
• Tharreau Didier, CIRAD-BIOS-UMR PHIM (FRA) ORCID: 0000-0003-3961-6120
• Zhou Bo, IRRI [International Rice Research Institute] (PHL) - auteur correspondant
• Wu Jun, Hunan Hybrid Rice Research Center (CHN) - auteur correspondant
• Kroj Thomas, INRAE (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609527/)

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