Comparative evolutionary genomics in insects

Feldmeyer Barbara, Bornberg-Bauer Erich, Dohmen Elias, Fouks Bertrand, Heckenhauer Jacqueline, Huylmans Ann Kathrin, Jones Alun R. C., Stolle Eckart, Harrison Mark C.. 2024. Comparative evolutionary genomics in insects. In : Comparative genomics: Methods and protocols. Setubal João Carlos (ed.), Stadler Peter F. (ed.), Stoye Jens (ed.). New York : Humana Press-Springer Nature, 473-514. (Methods in Molecular Biology, 2802) ISBN 978-1-0716-3838-5

Résumé : Genome sequencing quality, in terms of both read length and accuracy, is constantly improving. By combining long-read sequencing technologies with various scaffolding techniques, chromosome-level genome assemblies are now achievable at an affordable price for non-model organisms. Insects represent an exciting taxon for studying the genomic underpinnings of evolutionary innovations, due to ancient origins, immense species-richness, and broad phenotypic diversity. Here we summarize some of the most important methods for carrying out a comparative genomics study on insects. We describe available tools and offer concrete tips on all stages of such an endeavor from DNA extraction through genome sequencing, annotation, and several evolutionary analyses. Along the way we describe important insect-specific aspects, such as DNA extraction difficulties or gene families that are particularly difficult to annotate, and offer solutions. We describe results from several examples of comparative genomics analyses on insects to illustrate the fascinating questions that can now be addressed in this new age of genomics research.

Mots-clés libres : Génomique comparative, Insectes

Agences de financement hors UE : Deutsche Forschungsgemeinschaft, Deutscher Akademischer Austauschdienst

Auteurs et affiliations

• Feldmeyer Barbara, Senckenberg Biodiversity and Climate Research Centre (DEU)
• Bornberg-Bauer Erich, University of Münster (DEU)
• Dohmen Elias, University of Münster (DEU)
• Fouks Bertrand, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-3675-3499
• Heckenhauer Jacqueline, LOEWE Centre for Translational Biodiversity Genomics (DEU)
• Huylmans Ann Kathrin, Johannes Gutenberg Universität (DEU)
• Jones Alun R. C., University of Münster (DEU)
• Stolle Eckart, LIB (DEU)
• Harrison Mark C., University of Münster (DEU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/610292/)

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