A flow cytometry study of the binding and stimulation potential of inactivated Trypanosoma evansi toward dromedary camel leukocytes

Hussen Jama, Al-Jabr Omar A., Alkuwayti Mayyadah Abdullah, Alrabiah Noof Abdulrahman, Falemban Baraa, Alouffi Abdulaziz, Al Salim Waleed S., Kamyingkird Ketsarin, Desquesnes Marc. 2024. A flow cytometry study of the binding and stimulation potential of inactivated Trypanosoma evansi toward dromedary camel leukocytes. Pathogens, 13 (1), n.spéc. Parasitic Infections and Diseases of Domestic Animals:21, 15 p.

https://doi.org/10.3390/pathogens13010021

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Résumé : Surra, a wasting disease caused by Trypanosoma evansi, is one of the major animal health burdens in camel-rearing countries, imposing significant economic losses due to reduced fertility and high mortality rates. The present study used inactivated T. evansi (from the Card Agglutination Test for Trypanosomes/Trypanosoma evansi; CATT/T. evansi) and flow cytometry to investigate their binding and activation potential toward camel leukocyte subsets. Labeling T. evansi with propidium iodide (PI) enabled their flow cytometric enumeration and identification with forward scatter (FSC; indicative for cell size) and side scatter (SSC; indicative for cell internal complexity) characteristics that are comparable with values reported for Trypanosoma cruzi. The incubation of PI-labeled non-opsonized T. evansi with camel leukocyte populations revealed that camel monocytes have the highest potential to bind T. evansi, followed by granulocytes and lymphocytes. The identification of pattern recognition receptors (PRRs) on camel immune cells and the pathogen-associated molecular patterns (PAMPs) in T. evansi that are responsible for this different binding capacity requires further studies. Stimulation of camel neutrophils with Trypanosoma evansi induced shape change, reactive oxygen species (ROS) production, and neutrophil extracellular traps (NET)-formation. To ensure that T. evansi, in the parasite concentration used in this study, is not apoptotic or necrotic to camel leukocytes, we evaluated cell apoptosis and necrosis after stimulation with T. evansi. The results revealed no impact of T. evansi stimulation for 2 h on the cell viability of camel leukocytes. Subsequent work may focus on the diagnostic employment of labeled T. evansi and flow cytometry for the detection of anti-Trypanosoma antibodies in camel serum. In addition, more efforts should be deployed to investigate the host–pathogen interaction mechanisms and the escape mechanisms of T. evansi in camels. To complete these data, further studies using the living or freshly killed parasites could also be implemented in camels and/or horses.

Mots-clés Agrovoc : dromadaire, Trypanosoma evansi, surra, maladie des animaux, cytométrie en flux (cellules), santé animale, Trypanosoma, leucocyte, Trypanosoma cruzi, chameau

Mots-clés libres : Trypanosoma evansi, Camel, Phagocytosis, Neutrophils, NETosis, Flow cytometry

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Deanship of Scientific Research, King Faisal University

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