Isolation of Rift Valley fever virus from field specimens using cell culture approaches

Cetre-Sossah Catherine. 2024. Isolation of Rift Valley fever virus from field specimens using cell culture approaches. In : Rift valley fever virus: Methods and protocols. Lozach Pierre-Yves. New York : Humana Press, 27-34. (Methods in Molecular Biology) ISBN 978-1-0716-3925-2

Résumé : Several techniques have been developed to diagnose Rift Valley fever infection. Viral isolation is one of the most difficult techniques to apply but offers great opportunities for further research. It is useful, for example, for the development of an accurate diagnostic test suitable for screening for Rift Valley fever virus infection, specific treatments by testing known antiviral molecules that act on the replication cycle to assess their therapeutic or even prophylactic potential, therapeutic applications, and vaccine candidates. Understanding how the virus replicates and interacts with the host cell and organism and identifying biomarkers of infection or new targets for the development of treatments are made possible through field virus isolates. Biosafety level 3 conditions are a pre-requisite for viral isolation by a trained staff member. Here, we describe the procedure to isolate Rift Valley fever virus from field samples by cell culture.

Mots-clés libres : Isolation, Rift Valley fever virus, Cell culture

Auteurs et affiliations

• Cetre-Sossah Catherine, CIRAD-BIOS-UMR ASTRE (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/613261/)

Métriques

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