Sugarcane shoot formation in an improved temporary immersion system

Lorenzo José Carlos, Gonzalez Boris Luis, Escalona Maritza, Teisson Claude, Espinosa Patricia, Borroto Carlos. 1998. Sugarcane shoot formation in an improved temporary immersion system. Plant Cell, Tissue and Organ Culture, 54 : 197-200.

Résumé : A new protocol was established for sugarcane cv. C- 1051-73 shoot formation in a temporary immersion system. The two-step protocol involves shoot formation in 50 ml of culture medium per explant and 1.0 mg 1-1 paclobutrazol for 30 days followed by shoot elongation by exposure to 1.0 mg 1-1 gibberellic acid for 15 days. The multiplication rate was doubled in comparison with the conventional micropropagation protocol (Jiménez et al., 1995) and the cost has been reduced by 46%. Three additional sugarcane varieties have been micropropagated according to this new protocol and results are comparable. Temporary immersion -derived plants have also been compared with conventionally propagated plants in sugarcane fields for more than 9 months and their agricultural indicators of performance are similar. (Author's abstract)

Mots-clés Agrovoc : Saccharum, micropropagation, technique de culture, multiplication des plantes

Classification Agris : F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Lorenzo José Carlos, ISACA (CUB)
• Gonzalez Boris Luis, ISACA (CUB)
• Escalona Maritza, ISACA (CUB)
• Teisson Claude, CIRAD-AMIS-BIOTROP (FRA)
• Espinosa Patricia, ISACA (CUB)
• Borroto Carlos, ISACA (CUB)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/6368/)

Métriques

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