Chaïr Hâna, Kuhapituktum R., Attathom T., Pannetier Catherine, Attathom S.. 1997. Agrobacterium mediated transformation of Sri Sumrong 60, a Thai cotton variety. Kasetsart Journal. Natural Sciences, 31 (5) : 149-156.
Autre titre : Transformation par Agrobacterium de Thaï Sri Sumrong 60, variété de coton Thaï
Résumé : Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic crylA(b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of ctylA(b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60.
Mots-clés Agrovoc : Gossypium, variété, Agrobacterium tumefaciens, Embryogénèse somatique, cal, transfert de gène
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Chaïr Hâna, CIRAD-CA-COTON (THA)
- Kuhapituktum R., Kasetsart University (THA)
- Attathom T., Kasetsart University (THA)
- Pannetier Catherine, CIRAD-CA-COTON (FRA)
- Attathom S., Kasetsart University (THA)
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : CD_BR10003 [(Bibliothèque de Lavalette)] ; CD_BR8572 [(Bibliothèque de Lavalette)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/6577/)
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