Comparative flow cytometric estimation of nuclear DNA content in oil palm (Elaeis guineensis Jacq.) tissue cultures and seed-derived plants

Rival Alain, Beulé Thierry, Barré Philippe, Hamon Serge, Duval Yves, Noirot Michel. 1997. Comparative flow cytometric estimation of nuclear DNA content in oil palm (Elaeis guineensis Jacq.) tissue cultures and seed-derived plants. Plant Cell Reports, 16 (12) : 884-887.

Autre titre : Comparaison des estimations de l'ADN cellulaire contenu dans des cultures de tissus et des plantes de semence de palmier à huile Elaeis guineensis Jacq.) par analyse cytométrique par flux

Résumé : Flow cytometric analysis performed on two different crosses of #Dura x Pisifera# oil palm gave an accurate estimation of nuclear DNA content. The genome size of #Elaeis guineensis# was found to be 2C=3.76±0.09 pg and therefore ca. 3.4x10(9)bp. Embryogenic calli and plants showed the same ploidy level, but the measured 2C DNA values differed significantly. No variation in the ploidy level between three different types of calli originating from foliar explants, namely nodular compact callus, fast-growing callus and friable callus was observed. Since fast-growing callus (FGC), already identified as a source of 'mantled' phenotype variants, did not show any difference in their ploidy level, these results are consistent with the hypothesis of an epigenetic origin for this type of somaclonal variant.

Mots-clés Agrovoc : Elaeis guineensis, micropropagation, nombre chromosomique, variation somaclonale, embryon somatique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Rival Alain, CIRAD-CP-PALMIER (FRA) ORCID: 0000-0001-6304-3268
• Beulé Thierry, CIRAD-CP-PALMIER (FRA)
• Barré Philippe
• Hamon Serge
• Duval Yves, CIRAD-CP-PALMIER (FRA)
• Noirot Michel

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389576/)

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