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Bovine cytotoxic T-cell clones specific for cells infected with the protozoan parasite Theileria parva : Parasite strain specificity and class I major histocompatibility complex restriction

Goddeeris B.M., Morrison W.I., Teale A.J., Bensaïd Albert, Baldwin C.L.. 1986. Bovine cytotoxic T-cell clones specific for cells infected with the protozoan parasite Theileria parva : Parasite strain specificity and class I major histocompatibility complex restriction. Proceedings of the National Academy of Sciences of the United States of America, 83 (14) : pp. 5238-5242. ILRAD Laboratoire international pour la recherche sur les maladies animales, Nairobi, Kenya, 27 September 1987/1 October 1987.

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Autre titre : Des clones de cellules bovines cytotoxiques T dirigées contre les cellules infectées par le protozoaires T. parva : spécificité liée à la souche parasitaire et complexe majeur d'histocompatibilité de classe I

Matériel d'accompagnement : 1 micro-fiche numéro VT860221

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Economie-gestion; Psychologie-éthologie-ergonomie

Abstract : We present information on the specificity of three bovine cytotoxic T-cell clones reactive with lymphoblasts infected with the protozoan parasite Theileria parva. The clones were derived from peripheral blood mononuclear cells of an animal immunized with T. parva (Muguga stock), after five stimulations in vitro with an autologous parasitized cell line. The three clones belonged to the BoT8+ subset of T cells, which is similar to the human CD8+ T-cell subset. On the basis of analysis on a panel of infected target cells originating from cattle of different major histocompatibility complex (MHC) phenotypes, killing by all three clones was found to be restricted to targets bearing the class I MHC specificity KN104, which is defined by alloantiserum KNA104 and monoclonal antibody IL-A4. This class I MHC restriction was confirmed by blocking of target cell lysis with these antibodies and monoclonal antibody w6/32, which reacts with a nonpolymorphic determinant on bovine class I MHC molecules. The three clones were parasite strain specific, in that they did not kill cells of the appropriate MHC type infected with T. parva (Marikebuni stock). These findings, taken together with previous observations that immunization of cattle with T. parva (Muguga) does not provide protection against challenge with T. parva (Marikebuni), suggest that the cytotoxic T cells recognize a cell surface antigen that may be important in induction of immunity to the parasite.

Mots-clés Agrovoc : Bovin, Theilériose, Theileria parva, Lymphocyte, clone, Système mhc

Classification Agris : L20 - Animal ecology

Auteurs et affiliations

  • Goddeeris B.M.
  • Morrison W.I.
  • Teale A.J.
  • Bensaïd Albert
  • Baldwin C.L.

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/398017/)

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