Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium

Totté Philippe, McKeever D., Jongejan Frans, Barbet A., Mahan Suman M., Mwangi Duncan M., Bensaïd Albert. 1998. Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium. Annals of the New York Academy of Sciences, 849 : 155-160. Biennial Conference of the Society for Tropical Veterinary Medecine. 4, Montpellier, France, 5 Mai 1997/9 Mai 1997.

Autre titre : Analyse des réponses cellulaires des proteines naturelles et recombinantes de Cowdria ruminantium

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Histoire et philosophie des sciences; Histoire, histoire de l'art, archéologie; Psychologie-éthologie-ergonomie

Résumé : The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and yb T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were more than 90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only one cell line could be generated using MAP1 and it was composed of 85% yb T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.

Mots-clés Agrovoc : Ehrlichia ruminantium, réponse immunitaire, vaccin synthétique, immunisation, lymphocyte, protéine recombinante

Classification Agris : L72 - Organismes nuisibles des animaux

Auteurs et affiliations

• Totté Philippe, CIRAD-EMVT-SANTE ANIMALE (GLP)
• McKeever D.
• Jongejan Frans
• Barbet A.
• Mahan Suman M.
• Mwangi Duncan M.
• Bensaïd Albert, CIRAD-EMVT-SANTE ANIMALE (GLP)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/468058/)

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