The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice

Barakat Abdelali, Gallois Patrick, Raynal Martine, Mestre-Ortega Dominique, Sallaud Christophe, Guiderdoni Emmanuel, Delseny Michel, Bernardi Giorgio. 2000. The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice. FEBS Letters (471) : 161-164.

Résumé : Abstract Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed.

Mots-clés Agrovoc : Arabidopsis, Oryza sativa, plante transgénique, transfert de gène, localisation de gène

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Barakat Abdelali, Université de Perpignan (FRA)
• Gallois Patrick, Université de Perpignan (FRA)
• Raynal Martine, Université de Perpignan (FRA)
• Mestre-Ortega Dominique, Université de Perpignan (FRA)
• Sallaud Christophe, CIRAD-AMIS-BIOTROP (FRA)
• Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)
• Delseny Michel, Université de Perpignan (FRA)
• Bernardi Giorgio, Stazione Zoologica Anton Dohrn (ITA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/476847/)

Métriques

[ Page générée et mise en cache le 2024-05-04 ]