Immunochemical studies on gastric and intestinal digestion of soybean glycinin and bêta-conglycinin in vivo

Lallès Jean-Paul, Tukur Hussaini M., Salgado Paulo, Clare Mills E.N., Morgan Michael R.A., Quillien Laurence, Levieux Didier, Toullec René. 1999. Immunochemical studies on gastric and intestinal digestion of soybean glycinin and bêta-conglycinin in vivo. Journal of Agricultural and Food Chemistry, 47 (7) : 2797-2806.

Résumé : Two experiments were conducted to study gastric and small intestinal digestion of soybean glycinin and [bêta]-conglycinin in preruminant calves fed milk replacers containing a mixture of skim milk powder and antigenic heated soybean flour. In experiment 1, duodenal passage of immunoreactive [bêta]-conglycinin lasted for a much longer time after the morning meal than that of glycinin. Western blotting revealed the early abomasal outflow of glycinin subunits that associated nearly intact basic polypeptides to partially degraded acidic polypeptides. Intact [bêta]-conglycinin was evidenced at most sampling times. In experiment 2, intact basic glycinin (Mr = 21000) associated with partially digested acidic glycinin (7000 < Mr < 25000) was demonstrated in ileal digesta up to 8-10 h after the meal. [bêta]-Conglycinin immunoreactivity could not be evidenced by Western blotting in ileal digesta.

Auteurs et affiliations

• Lallès Jean-Paul, INRA (FRA)
• Tukur Hussaini M., INRA (FRA)
• Salgado Paulo, INRA (FRA)
• Clare Mills E.N., Institut of Food Research (GBR)
• Morgan Michael R.A., Institut of Food Research (GBR)
• Quillien Laurence, Institut of Food Research (GBR)
• Levieux Didier, INRA (FRA)
• Toullec René, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512540/)

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