Agritrop
Home

Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes

Royer Monique, Costet Laurent, Vivien Eric, Bes Martine, Cousin Arnaud, Damais Anne, Pieretti Isabelle, Savin Alexandre, Megessier Sandrine, Viard Magali, Frutos Roger, Gabriel Dean W., Rott Philippe. 2004. Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes. Molecular Plant-Microbe Interactions, 17 (4) : pp. 414-427.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
document_519067.pdf

Télécharger (545kB)

Type d'url non précisé : http://www.apsnet.org/mpmi/pdfs/2004/0212-01R.pdf

Abstract : Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin called albicidin. We report here sequencing and homology analysis of the major gene cluster, XALB1 (55,839 bp), and a second, smaller region, XALB2 (2,986 bp), involved in albicidin biosynthesis. XALB1 contains 20 open reading frames, including i) three large genes with a modular architecture characteristic of polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and ii) several putative modifying, regulatory, and resistance genes. Sequencing and complementation studies of six albicidin-defective mutants enabled us to confirm the involvement of the three PKS and NRPS genes encoded by XALB1 in albicidin production. XALB2 contains only one gene that is required for post-translational activation of PKS and NRPS enzymes, confirming the involvement of these enzymes in albicidin biosynthesis. In silico analysis of these three PKS or NRPS enzymes allowed us to propose a model for the albicidin backbone assembly and to gain insight into the structural features of this pathotoxin. This is the first description of a complete mixed PKS-NRPS gene cluster for toxin production in the genus Xanthomonas. (Résumé d'auteur)

Classification Agris : H20 - Plant diseases

Auteurs et affiliations

  • Royer Monique, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Costet Laurent, CIRAD-CA-Canne à sucre (REU) ORCID: 0000-0003-3199-2885
  • Vivien Eric, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Bes Martine, CIRAD-AMIS-BIOTROP (FRA)
  • Cousin Arnaud, CIRAD-AMIS-UMR BGPI (FRA)
  • Damais Anne, CIRAD-AMIS-UMR BGPI (FRA)
  • Pieretti Isabelle, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Savin Alexandre, CIRAD-AMIS-UMR BGPI (FRA)
  • Megessier Sandrine, CIRAD-AMIS-UMR BGPI (FRA)
  • Viard Magali, CIRAD-AMIS-UMR BGPI (FRA)
  • Frutos Roger, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Gabriel Dean W., CIRAD-AMIS-UMR BGPI (FRA)
  • Rott Philippe, CIRAD-CA-Canne à sucre (FRA) ORCID: 0000-0001-6085-6159

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/519067/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-06-20 ]