Infolding and refolding of active apple polyphenol oxidase

Mari Stéphane, Marquès Laurence, Breton Frédéric, Karamanos Yannis, Macheix Jean-Jacques. 1998. Infolding and refolding of active apple polyphenol oxidase. Phytochemistry, 49 (5) : 1213-1217.

Résumé : For the first time, unfolding (6 M guanidine) and refolding of partially proteolysed purified polyphenol oxidase (PPOr) was achieved, with 88% of activity recovered. Optimal refolding conditions consisted in stepwise dialysis of guanidine treated extracts, the dialysis buffers containing 1 M (NH4)2SO4 and 100 [mu]M CuSO4. However, CuSO4 had limited effect on the recovering of PPOr activity, whereas (NH4)2SO4 was essential. Concerning the PPO tertiary structure, denaturing conditions (combinations of boiling and reducing agent) used on SDS-PAGE have shown (i) a compact tertiary structure and (ii) the presence of disulfide bonds in PPOr, accounting for the shift between 27 and 41 kDa, and 41 and 42 kDa, respectively. Resistance to proteolytic cleavage was used to study the conformational changes induced by the denaturing treatments. Folded PPOr was resistant to further proteolysis whereas unfolded PPO was totally digested, indicating the role of tertiary structure of PPOr in the resistance to proteases.

Auteurs et affiliations

• Mari Stéphane, UM2 (FRA)
• Marquès Laurence, UM2 (FRA)
• Breton Frédéric, CIRAD-CP-HEVEA (FRA) ORCID: 0000-0002-6853-2623
• Karamanos Yannis, Université d'Artois (FRA)
• Macheix Jean-Jacques, UM2 (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525272/)

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