Analise da expressão de genes candidatos para a tolerância a seca em folhas de clones de Coffea canephora Var. Cornillon, caracterizados fisiologiamente

Marraccini Pierre, Da Silva Vânia A., Elbelt Sonia, Guimarães Breno L.S., Loureiro Marcelo E., Damatta Fabio M., Ferrão Maria Amélia G., Da Fonseca Aymbiré F.A., Da Silva Felipe, Andrade Alan Carvalho. 2007. Analise da expressão de genes candidatos para a tolerância a seca em folhas de clones de Coffea canephora Var. Cornillon, caracterizados fisiologiamente. In : 5° Simposio de Pesquisa dos Cafés do Brasil, Aguas de lindoia, Brasil, May 7-11, 2007. EMBRAPA. s.l. : s.n., 5 p. Simposio de Pesquisa dos Cafés do Brasil. 5, Aguas de Lindoia, Brésil, 7 Mai 2007/11 Mai 2007.

Titre anglais : Gene expression analysis of candidate genes for drought tolerance in leaves from clones of Coffea canephora Var. Cornillon phyisologically characterized

Résumé : Candidate genes involved in coffee drought tolerance were selected by in silico analysis (Electronic Northern) of SH2 and SH3 cDNA libraries of the Coffee Genome database. Eighteen "contigs" with in silico differential expression were identified and characterized by Northern-blot experiments using total RNA extracted from leaves of drought tolerant and susceptible clones of Coffea canephora var. Conillon grown in greenhouse under controlled conditions. Physiological analyses showed that the clone 22 (drought susceptible) presented faster decrease of leaf water potential than resistant ones that was attributed to its higher stomatal conductance. Below -3.0MPa, this clone also showed lower photosynthetic activity correlated with elevated oxidative stress. For most of the candidate genes selected by "Electronic Northern" approach, differential gene expression was confirmed by conventional Northern-blot analyses. Interestingly, some of them also presented differential expression patterns between the four clones tested (drought susceptible vs. drought tolerant). Research perspectives are discussed based on these results.

Classification Agris : F30 - Génétique et amélioration des plantes
H50 - Troubles divers des plantes

Auteurs et affiliations

• Marraccini Pierre, CIRAD-BIOS-UMR DAP (BRA) ORCID: 0000-0001-7637-6811
• Da Silva Vânia A., UFV (BRA)
• Elbelt Sonia, EMBRAPA (BRA)
• Guimarães Breno L.S., UFV (BRA)
• Loureiro Marcelo E., UFV (BRA)
• Damatta Fabio M., UFV (BRA)
• Ferrão Maria Amélia G., INCAPER (BRA)
• Da Fonseca Aymbiré F.A., INCAPER (BRA)
• Da Silva Felipe, EMBRAPA (BRA)
• Andrade Alan Carvalho, EMBRAPA (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540487/)

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